The Jensen Lab is fully equipped with state-of-the-art equipment for high-throughput CryoEM
FEI Tecnai Polara (F30)
The heart and soul of the Jensen Lab is our 300 kV cryo-electron microscope commonly referred to as the Polara. It is equipped with a 4k x 4k Gatan K2 direct electron detector positioned behind a Gatan GIF energy filter to provide the best possible images of whole cryo-preserved cells.
FEI Tecnai (T12)
The T12 is a 120 kV workhorse in the lab that is used for negative stain imaging, tomography of tissue sections and screening cryo-preserved specimens before putting them in the Polara. It is equipped with a Gatan CCD camera.
FEI Vitrobots (Mark III and IV)
A pair of Vitrobots ensures that excellent cryo-preservation is always routinely achieved.
This tried and true glow discharger is used to prepare a hydrophyllic surface on our EM grids before sample application and plunge freezing with the Vitrobot.