Publications – Microbial Cell Biology

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2017

Journal of Bacteriology

Uncharacterized bacterial structures revealed by electron cryotomography

Dobro MJ, Oikonomou CM, Piper A, Cohen J, Guo K, Jensen T, Tadayon J, Donermeyer J, Park Y, Solis BA, Kjaer A, Jewett AI, McDowall AW, Chen S, Chang Y-W, Shi J, Subramanian P, Iancu CV, Li Z, Briegel A, Tocheva EI, Pilhofer M, Jensen GJ

Subcellular Biochemistry

The Structure, Function and Roles of the Archaeal ESCRT Apparatus

Samson RY, Dobro MJ, Jensen GJ, Bell SD

The EMBO Journal

Short FtsZ filaments can drive asymmetric cell envelope constriction at the onset of bacterial cytokinesis

Yao Q, Jewett AI, Chang Y-W, Oikonomou CM, Beeby M, Iancu CV, Briegel A, Ghosal D, Jensen GJ

Science

Giant viruses with an expanded complement of translation system components

Schulz F, Yutin N, Ivanova NN, Ortega DR, Lee TK, Vierheilig J, Daims H, Horn M, Wagner M, Jensen GJ, Kyrpides NC, Koonin EV, Woyke T

EMBO Reports

Structure of the Legionella Dot/Icm type IV secretion system in situ by electron cryotomography

Ghosal D, Chang Y-W, Jeong KC, Vogel JP, Jensen GJ

bioRxiv

Structure of the archaellar motor and associated cytoplasmic cone in Thermococcus kodakaraensis

Briegel A, Oikonomou CM, Chang Y-W, Kjaer A, Huang AN, Kim KW, Ghosal D, Gunsalus RP, Jensen GJ

Nature Microbiology

Architecture of the Vibrio cholerae toxin-coregulated pilus machine revealed by electron cryotomography

Chang Y-W, Kjaer A, Ortega DR, Kovacikova G, Sutherland JA, Rettberg LA, Taylor RK, Jensen GJ

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2016

Molecular Microbiology

Dynamics of the peptidoglycan biosynthetic machinery in the stalked budding bacterium Hyphomonas neptunium

Cserti E, Rosskopf S, Chang Y-W, Eisheuer S, Selter L, Shi J, Regh C, Koert U, Jensen GJ, Thanbichler M

Molecular Microbiology

Starvation and recovery in the deep-sea methanotroph Methyloprofundus sedimenti

Tavormina PL, Kellermann MY, Antony CP, Tocheva E, Dalleska N, Jensen AJ, Valentine DL, Hinrichs KU, Jensen G, Dubilier N, Orphan VJ

PNAS

Diverse high-torque bacterial flagellar motors assemble wider stator rings using a conserved protein scaffold

Beeby M, Ribardo DA, Brennan CA, Ruby EG, Jensen GJ, Hendrixson DR

Science

Architecture of the type IVa pilus machine

Chang Y-W, Rettberg LA, Treuner-Lange A, Iwasa J, Sogaard-Andersen L, Jensen GJ

Nature Reviews Microbiology

A new view into prokaryotic cell biology from electron cryotomography

Oikonomou CM, Chang Y-W, Jensen GJ

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2015

Environmental Microbiology Reports

Structural conservation of chemotaxis machinery across Archaea and Bacteria

Briegel A, Ortega DR, Huang AN, Oikonomou CM, Gunsalus RP, Jensen GJ

Journal of Biomolecular Screening

Streptomyces: a screening tool for bacterial cell division inhibitors

Jani C, Tocheva EI, McAuley S, Craney A, Jensen GJ, Nodwell J

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2014

Microbiology Open

Ultrastructure and complex polar architecture of the human pathogen Campylobacter jejuni

Müller A, Beeby M, McDowall AW, Chow J, Jensen GJ, Clemons WM Jr.

Nature Methods

Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography

Chang YW, Chen S, Tocheva EI, Treuner-Lange A, Löbach S, Søgaard-Andersen L, Jensen GJ

eLife

Structure of bacterial cytoplasmic chemoreceptor arrays and implications for chemotactic signaling

Briegel A, Ladinsky MS, Oikonomou C, Jones CW, Harris MJ, Fowler DJ, Chang YW, Thompson LK, Armitage JP, Jensen GJ

PLOS Computational Biology

Escherichia coli peptidoglycan structure and mechanics as predicted by atomic-scale simulations

Gumbart JC, Beeby M, Jensen GJ, Roux B

Biochemistry

New insights into bacterial chemoreceptor array structure and assembly from electron cryotomography

Briegel A, Wong ML, Hodges HL, Oikonomou CM, Piasta KN, Harris MJ, Fowler DJ, Thompson LK, Falke JJ, Kiessling LL, Jensen GJ

Journal of Bacteriology

Structure and expression of propanediol utilization microcompartments in Acetonema longum

Tocheva EI, Matson EG, Cheng SN, Chen WG, Leadbetter JR, Jensen GJ

Science

Marine tubeworm metamorphosis induced by arrays of bacterial phage tail-like structures

Shikuma NJ, Pilhofer M, Weiss GL, Hadfield MG, Jensen GJ, Newman DK

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2013

Nature Communications

Discovery of chlamydial peptidoglycan reveals bacteria with murein sacculi but without FtsZ

Pilhofer M, Aistleitner K, Biboy J, Gray J, Kuru E, Hall E, Brun YV, VanNieuwenhze MS, Vollmer W, Horn M, Jensen GJ

Environmental Microbiology

Architecture and host interface of environmental chlamydiae revealed by electron cryotomography

Pilhofer M, Aistleitner K, Ladinsky MS, König L, Horn M, Jensen GJ

Journal of Bacteriology

Polyphosphate storage during sporulation in the gram-negative bacterium Acetonema longum

Tocheva EI, Dekas AE, McGlynn SE, Morris D, Orphan VJ, Jensen GJ

Molecular Microbiology

The mobility of two kinase domains in the Escherichia coli chemoreceptor array varies with signalling state

Briegel A, Ames P, Gumbart JC, Oikonomou CM, Parkinson JS, Jensen GJ

Molecular Biology of the Cell

Electron cryotomography of ESCRT assemblies and dividing Sulfolobus cells suggests that spiraling filaments are involved in membrane scission

Dobro MJ, Samson RY, Yu Z, McCullough J, Ding HJ, Chong PL, Bell SD, Jensen GJ

Molecular Microbiology

Architecture and assembly of the Gram-positive cell wall

Beeby M, Gumbart JC, Roux B, Jensen GJ

Molecular Microbiology

Peptidoglycan transformations during Bacillus subtilis sporulation

Tocheva EI, López-Garrido J, Hughes HV, Fredlund J, Kuru E, Vannieuwenhze MS, Brun YV, Pogliano K, Jensen GJ

Nature Structural and Molecular Biology

Architecture of the major component of the type III secretion system export apparatus

Abrusci P, Vergara-Irigaray M, Johnson S, Beeby MD, Hendrixson DR, Roversi P, Friede ME, Deane JE, Jensen GJ, Tang CM, Lea SM

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2012

Cell

General protein diffusion barriers create compartments within bacterial cells

Schlimpert S, Klein EA, Briegel A, Hughes V, Kahnt J, Bolte K, Maier UG, Brun YV, Jensen GJ, Gitai Z, Thanbichler M

Current Opinion in Cell Biology

The bacterial cytoskeleton: more than twisted filaments

Pilhofer M, Jensen GJ

Genes and Development

A multidomain hub anchors the chromosome segregation and chemotactic machinery to the bacterial pole

Yamaichi Y, Bruckner R, Ringgaard S, Möll A, Cameron DE, Briegel A, Jensen GJ, Davis BM, Waldor MK

Nature

Type VI secretion requires a dynamic contractile phage tail-like structure

Basler M, Pilhofer M, Henderson GP, Jensen GJ, Mekalanos JJ

Journal of Bacteriology

Growth and localization of polyhydroxybutyrate granules in Ralstonia eutropha

Beeby M, Cho M, Stubbe J, Jensen GJ

Quarterly Reviews of Biophysics

Electron Tomography of Cells

Gan L, Jensen GJ

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2011

PLoS Biology

Microtubules in bacteria: Ancient tubulins build a five-protofilament homolog of the eukaryotic cytoskeleton

Pilhofer M, Ladinsky MS, McDowall AW, Petroni G, Jensen GJ

Molecular Microbiology

Activated chemoreceptor arrays remain intact and hexagonally packed

Briegel A, Beeby M, Thanbichler M, Jensen GJ

Cell

Peptidoglycan remodeling and conversion of an inner membrane into an outer membrane during sporulation

Tocheva EI, Matson EG, Morris DM, Moussavi F, Leadbetter JR, Jensen GJ

PLoS One

Nanopods: a new bacterial structure and mechanism for deployment of outer membrane vesicles

Shetty A, Chen S, Tocheva EI, Jensen GJ, Hickey WJ

The EMBO Journal

Structural diversity of bacterial flagellar motors

Chen S, Beeby M, Murphy GE, Leadbetter JR, Hendrixson DR, Briegel A, Li Z, Shi J, Tocheva EI, Müller A, Dobro MJ, Jensen GJ

PNAS

Alternative mechanism for bacteriophage adsorption to the motile bacterium Caulobacter crescentus

Guerrero-Ferreira RC, Viollier PH, Ely B, Poindexter JS, Georgieva M, Jensen GJ, Wright ER

Biochemical and Biophysical Research Communications

Long helical filaments are not seen encircling cells in electron cryotomograms of rod-shaped bacteria

Swulius MT, Chen S, Jane Ding H, Li Z, Briegel A, Pilhofer M, Tocheva EI, Lybarger SR, Johnson TL, Sandkvist M, Jensen GJ

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2010

Structure

In vivo assembly of an archaeal virus studied with whole-cell electron cryotomography

Fu CY, Wang K, Gan L, Lanman J, Khayat R, Young MJ, Jensen GJ, Doerschuk PC, Johnson JE

Methods in Cell Biology

Bacterial TEM: new insights from cryo-microscopy

Pilhofer M, Ladinsky MS, McDowall AW, Jensen GJ

Nature Cell Biology

The metabolic enzyme CTP synthase forms cytoskeletal filaments

Ingerson-Mahar M, Briegel A, Werner JN, Jensen GJ, Gitai Z

Cold Spring Harbor Perspectives in Biology

Electron cryotomography

Tocheva EI, Li Z, Jensen GJ

Molecular Microbiology

DipM, a new factor required for peptidoglycan remodelling during cell division in Caulobacter crescentus

Möll A, Schlimpert S, Briegel A, Jensen GJ, Thanbichler M

JoVE

Electron cryotomography of bacterial cells

Chen S, McDowall A, Dobro MJ, Briegel A, Ladinsky M, Shi J, Tocheva EI, Beeby M, Pilhofer M, Ding HJ, Li Z, Gan L, Morris DM, Jensen GJ

Journal of Bacteriology

Mutations in the Lipopolysaccharide biosynthesis pathway interfere with crescentin-mediated cell curvature in Caulobacter crescentus

Cabeen MT, Murolo MA, Briegel A, Bui NK, Vollmer W, Ausmees N, Jensen GJ, Jacobs-Wagner C

The EMBO Journal

Bactofilins, a ubiquitous class of cytoskeletal proteins mediating polar localization of a cell wall synthase in Caulobacter crescentus

Kühn J, Briegel A, Mörschel E, Kahnt J, Leser K, Wick S, Jensen GJ, Thanbichler M

Journal of Molecular Biology

Organization, structure, and assembly of alpha-carboxysomes determined by electron cryotomography of intact cells

Iancu CV, Morris DM, Dou Z, Heinhorst S, Cannon GC, Jensen GJ

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2009

PNAS

Universal architecture of bacterial chemoreceptor arrays

Briegel A, Ortega DR, Tocheva EI, Wuichet K, Li Z, Chen S, Müller A, Iancu CV, Murphy GE, Dobro MJ, Zhulin IB, Jensen GJ

Current Opinion in Microbiology

Electron cryotomography: a new view into microbial ultrastructure

Li Z, Jensen GJ

Science

Cell biology. Protein filaments caught in the act

Jensen GJ

Comment on

Electron cryomicroscopy of E. coli reveals filament bundles involved in plasmid DNA segregation

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2008

Molecular Microbiology

Location and architecture of the Caulobacter crescentus chemoreceptor array

Briegel A, Ding HJ, Li Z, Werner J, Gitai Z, Dias DP, Jensen RB, Jensen GJ

Annual Review of Biochemistry

Toward a biomechanical understanding of whole bacterial cells

Morris DM, Jensen GJ

Molecular Microbiology

Novel ultrastructures of Treponema primitia and their implications for motility

Murphy GE, Matson EG, Leadbetter JR, Berg HC, Jensen GJ

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2007

Biotechniques

Electron cryotomography

Murphy GE, Jensen GJ

Journal of Molecular Biology

The structure of isolated Synechococcus strain WH8102 carboxysomes as revealed by electron cryotomography

Iancu CV, Ding HJ, Morris DM, Dias DP, Gonzales AD, Martino A, Jensen GJ

Current Opinion in Structural Biology

How electron cryotomography is opening a new window onto prokaryotic ultrastructure

Jensen GJ, Briegel A

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2006

Molecular Microbiology

Multiple large filament bundles observed in Caulobacter crescentus by electron cryotomography

Briegel A, Dias DP, Li Z, Jensen RB, Frangakis AS, Jensen GJ

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2005