Science
The stress-sensing domain of activated IRE1α forms helical filaments in narrow ER membrane tubes
Tran N-H*, Carter SD*, De Maziére A, Ashkenazi A, Klumperman J, Walter P+, Jensen GJ+
Science Advances
A cryo-electron tomography workflow reveals protrusion-mediated shedding on injured plasma membrane
Mageswaran SK, Yang WY+, Chakrabarty Y, Oikonomou CM, Jensen GJ+
Science Advances
Visualizing insulin vesicle neighborhoods in β cells by cryo-electron tomography
Zhang X, Carter SD, Singla J, White KL, Butler PC, Stevens RC+, Jensen GJ+
PNAS
Correlated cryogenic fluorescence microscopy and electron cryo-tomography shows that exogenous TRIM5α can form hexagonal lattices or autophagy aggregates in vivo
Carter SD, Mamede JI, Hope TJ, Jensen GJ
Nature Communications
CryoEM structure of the Type IVa pilus secretin required for natural competence in Vibrio cholerae
Weaver SJ, Ortega DR, Sazinsky MH, Dalia TN, Dalia AB, Jensen GJ
Nature Communications
Repurposing a chemosensory macromolecular machine
Ortega DR, Yang W, Subramanian P, Mann P, Kjaer A, Chen S, Watts KJ, Pirbadian A, Collins DA, Kooger R, Kalyuzhnaya MG, Ringgaard S, Briegel A+, Jensen GJ+
Nature Microbiology
In vivo structure of the Legionella type II secretion system by electron cryotomography
Ghosal D, Kim KW, Zheng H, Kaplan M, Truchan HK, Lopez AE, McIntire IE, Vogel JP, Cianciotto NP, Jensen GJ
EMBO Journal
In situ imaging of the bacterial flagellar motor disassembly and assembly processes
Kaplan M, Subramanian P, Ghosal D, Oikonomou CM, Pirbadian S, Starwalt-Lee R, Mageswaran SK, Ortega DR, Gralnick JA, El-Naggar MY, Jensen GJ
Nature Microbiology
Molecular architecture, polar targeting and biogenesis of the Legionella Dot/Icm T4SS
Ghosal D*, Jeong KC*, Chang Y-W, Gyore J, Teng L, Gardner A, Vogel JP+, Jensen GJ+
Nature Communications
Simulations suggest a constrictive force is required for Gram-negative bacterial cell division
Nguyen LT, Oikonomou CM, Ding HJ, Kaplan M, Yao Q, Chang Y-W, Beeby M, Jensen GJ
Journal of Structural Biology
Rapid tilt-series acquisition for electron cryotomography
Chreifi G*, Chen S*, Metskas LA, Kaplan M, Jensen GJ
Cell
Programmed secretion arrest and receptor-triggered toxin export during antibacterial contact-dependent growth inhibition
Ruhe ZC*, Subramanian P*, Song K*, Nguyen JY, Stevens TA, Low DA, Jensen GJ+, Hayes CS+
Cell Reports
In vivo structures of the Helicobacter pylori cag type IV secretion system
Chang Y-W*, Shaffer CL*, Rettberg LA, Ghosal D, Jensen GJ
PNAS
Ultrastructure of Shewanella oneidensis MR-1 nanowires revealed by electron cryotomography
Subramanian P*, Pirbadian S*, El-Naggar MY+, Jensen GJ+
PNAS
Structure of the fission yeast actomyosin ring during constriction
Swulius MT*, Nguyen LT*, Ladinsky MS, Ortega DR, Aich S, Mishra M, Jensen GJ
EMBO Reports
In vivo structures of an intact type VI secretion system revealed by electron cryotomography
Chang Y-W, Rettberg LA, Ortega DR, Jensen GJ
The EMBO Journal
Short FtsZ filaments can drive asymmetric cell envelope constriction at the onset of bacterial cytokinesis
Yao Q, Jewett AI, Chang Y-W, Oikonomou CM, Beeby M, Iancu CV, Briegel A, Ghosal D, Jensen GJ
Nature Microbiology
Architecture of the Vibrio cholerae toxin-coregulated pilus machine revealed by electron cryotomography
Chang Y-W, Kjaer A, Ortega DR, Kovacikova G, Sutherland JA, Rettberg LA, Taylor RK, Jensen GJ
Science
Architecture of the type IVa pilus machine
Chang Y-W, Rettberg LA, Treuner-Lange A, Iwasa J, Sogaard-Andersen L, Jensen GJ
PNAS
Coarse-grained simulations of bacterial cell wall growth reveal that local coordination alone can be sufficient to maintain rod shape
Nguyen LT, Gumbart JC, Beeby M, Jensen GJ
Nature Methods
Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography
Chang YW, Chen S, Tocheva EI, Treuner-Lange A, Löbach S, Søgaard-Andersen L, Jensen GJ
Science
Marine tubeworm metamorphosis induced by arrays of bacterial phage tail-like structures
Shikuma NJ*, Pilhofer M*, Weiss GL, Hadfield MG+, Jensen GJ+, Newman DK+
Nature Communications
Discovery of chlamydial peptidoglycan reveals bacteria with murein sacculi but without FtsZ
Pilhofer M*, Aistleitner K*, Biboy J, Gray J, Kuru E, Hall E, Brun YV, VanNieuwenhze MS, Vollmer W, Horn M+, Jensen GJ+
Molecular Biology of the Cell
Electron cryotomography of ESCRT assemblies and dividing Sulfolobus cells suggests that spiraling filaments are involved in membrane scission
Dobro MJ, Samson RY, Yu Z, McCullough J, Ding HJ, Chong PL, Bell SD, Jensen GJ
Molecular Microbiology
Architecture and assembly of the Gram-positive cell wall
Beeby M, Gumbart JC, Roux B, Jensen GJ
Nature Structural and Molecular Biology
Architecture of the major component of the type III secretion system export apparatus
Abrusci P, Vergara-Irigaray M, Johnson S, Beeby MD, Hendrixson DR, Roversi P, Friede ME, Deane JE, Jensen GJ, Tang CM, Lea SM
Journal of Molecular Biology
Unclosed HIV-1 capsids suggest a curled sheet model of assembly
Yu Z*, Dobro MJ*, Woodward CL, Levandovsky A, Danielson CM, Sandrin V, Shi J, Aiken C, Zandi R, Hope TJ, Jensen GJ
Cell
General protein diffusion barriers create compartments within bacterial cells
Schlimpert S*, Klein EA*, Briegel A, Hughes V, Kahnt J, Bolte K, Maier UG, Brun YV, Jensen GJ, Gitai Z, Thanbichler M
Journal of Bacteriology
The helical MreB cytoskeleton in Escherichia coli MC1000/pLE7 is an artifact of the N-Terminal yellow fluorescent protein tag
Swulius MT, Jensen GJ
Nature
Type VI secretion requires a dynamic contractile phage tail-like structure
Basler M*, Pilhofer M*, Henderson GP, Jensen GJ+, Mekalanos JJ+
PNAS
Bacterial chemoreceptor arrays are hexagonally packed trimers of receptor dimers networked by rings of kinase and coupling proteins
Briegel A*, Li X*, Bilwes AM, Hughes KT, Jensen GJ+, Crane BR+
PLoS Biology
Microtubules in bacteria: Ancient tubulins build a five-protofilament homolog of the eukaryotic cytoskeleton
Pilhofer M+, Ladinsky MS, McDowall AW, Petroni G, Jensen GJ+
Molecular Microbiology
Activated chemoreceptor arrays remain intact and hexagonally packed
Briegel A, Beeby M, Thanbichler M, Jensen GJ
Cell
Peptidoglycan remodeling and conversion of an inner membrane into an outer membrane during sporulation
Tocheva EI, Matson EG, Morris DM, Moussavi F, Leadbetter JR, Jensen GJ
The EMBO Journal
Structural diversity of bacterial flagellar motors
Chen S*, Beeby M*, Murphy GE, Leadbetter JR, Hendrixson DR, Briegel A, Li Z, Shi J, Tocheva EI, Müller A, Dobro MJ, Jensen GJ
PNAS
Alternative mechanism for bacteriophage adsorption to the motile bacterium Caulobacter crescentus
Guerrero-Ferreira RC, Viollier PH, Ely B, Poindexter JS, Georgieva M, Jensen GJ, Wright ER
Biochemical and Biophysical Research Communications
Long helical filaments are not seen encircling cells in electron cryotomograms of rod-shaped bacteria
Swulius MT, Chen S, Jane Ding H, Li Z, Briegel A, Pilhofer M, Tocheva EI, Lybarger SR, Johnson TL, Sandkvist M, Jensen GJ
Nature Cell Biology
The metabolic enzyme CTP synthase forms cytoskeletal filaments
Ingerson-Mahar M, Briegel A, Werner JN, Jensen GJ, Gitai Z
Journal of Molecular Biology
Organization, structure, and assembly of alpha-carboxysomes determined by electron cryotomography of intact cells
Iancu CV*, Morris DM*, Dou Z, Heinhorst S, Cannon GC, Jensen GJ
Microscopy and Microanalysis
An improved cryogen for plunge freezing
Tivol WF+, Briegel A, Jensen GJ
Molecular Microbiology
Novel ultrastructures of Treponema primitia and their implications for motility
Murphy GE, Matson EG, Leadbetter JR, Berg HC, Jensen GJ
The EMBO Journal
The structure of FtsZ filaments in vivo suggests a force-generating role in cell division
Li Z, Trimble MJ, Brun YV, Jensen GJ
PLoS One
3-D ultrastructure of O. tauri: electron cryotomography of an entire eukaryotic cell
Henderson GP, Gan L, Jensen GJ
The EMBO Journal
Electron cryotomography of immature HIV-1 virions reveals the structure of the CA and SP1 Gag shells
Wright ER*, Schooler JB*, Ding HJ, Kieffer C, Fillmore C, Sundquist WI, Jensen GJ
Nature
In situ structure of the complete Treponema primitia flagellar motor
Murphy GE, Leadbetter JR, Jensen GJ
Molecular Microbiology
Three-dimensional structure of Mycoplasma pneumoniae’s attachment organelle and a model for its role in gliding motility
Henderson GP, Jensen GJ
Science
Magnetosomes are cell membrane invaginations organized by the actin-like protein MamK
Komeili A*, Li Z*, Newman DK, Jensen GJ
Journal of Molecular Biology
Three-dimensional structure of HIV-1 virus-like particles by electron cryotomography
Benjamin J, Ganser-Pornillos BK, Tivol WF, Sundquist WI, Jensen GJ