Publications – Featured Papers




The stress-sensing domain of activated IRE1α forms helical filaments in narrow ER membrane tubes

Tran N-H*, Carter SD*, De Maziére A, Ashkenazi A, Klumperman J, Walter P+, Jensen GJ+

Science Advances

A cryo-electron tomography workflow reveals protrusion-mediated shedding on injured plasma membrane

Mageswaran SK, Yang WY+, Chakrabarty Y, Oikonomou CM, Jensen GJ+



Science Advances

Visualizing insulin vesicle neighborhoods in β cells by cryo-electron tomography

Zhang X, Carter SD, Singla J, White KL, Butler PC, Stevens RC+, Jensen GJ+

Nature Communications

CryoEM structure of the Type IVa pilus secretin required for natural competence in Vibrio cholerae

Weaver SJ, Ortega DR, Sazinsky MH, Dalia TN, Dalia AB, Jensen GJ

Nature Communications

Repurposing a chemosensory macromolecular machine

Ortega DR, Yang W, Subramanian P, Mann P, Kjaer A, Chen S, Watts KJ, Pirbadian A, Collins DA, Kooger R, Kalyuzhnaya MG, Ringgaard S, Briegel A+, Jensen GJ+



Nature Microbiology

In vivo structure of the Legionella type II secretion system by electron cryotomography

Ghosal D, Kim KW, Zheng H, Kaplan M, Truchan HK, Lopez AE, McIntire IE, Vogel JP, Cianciotto NP, Jensen GJ

EMBO Journal

In situ imaging of the bacterial flagellar motor disassembly and assembly processes

Kaplan M, Subramanian P, Ghosal D, Oikonomou CM, Pirbadian S, Starwalt-Lee R, Mageswaran SK, Ortega DR, Gralnick JA, El-Naggar MY, Jensen GJ

Nature Microbiology

Molecular architecture, polar targeting and biogenesis of the Legionella Dot/Icm T4SS

Ghosal D*, Jeong KC*, Chang Y-W, Gyore J, Teng L, Gardner A, Vogel JP+, Jensen GJ+

Nature Communications

Simulations suggest a constrictive force is required for Gram-negative bacterial cell division

Nguyen LT, Oikonomou CM, Ding HJ, Kaplan M, Yao Q, Chang Y-W, Beeby M, Jensen GJ

Journal of Structural Biology

Rapid tilt-series acquisition for electron cryotomography

Chreifi G*, Chen S*, Metskas LA, Kaplan M, Jensen GJ




Programmed secretion arrest and receptor-triggered toxin export during antibacterial contact-dependent growth inhibition

Ruhe ZC*, Subramanian P*, Song K*, Nguyen JY, Stevens TA, Low DA, Jensen GJ+, Hayes CS+

Cell Reports

In vivo structures of the Helicobacter pylori cag type IV secretion system

Chang Y-W*, Shaffer CL*, Rettberg LA, Ghosal D, Jensen GJ


Ultrastructure of Shewanella oneidensis MR-1 nanowires revealed by electron cryotomography

Subramanian P*, Pirbadian S*, El-Naggar MY+, Jensen GJ+


Structure of the fission yeast actomyosin ring during constriction

Swulius MT*, Nguyen LT*, Ladinsky MS, Ortega DR, Aich S, Mishra M, Jensen GJ



The EMBO Journal

Short FtsZ filaments can drive asymmetric cell envelope constriction at the onset of bacterial cytokinesis

Yao Q, Jewett AI, Chang Y-W, Oikonomou CM, Beeby M, Iancu CV, Briegel A, Ghosal D, Jensen GJ

Nature Microbiology

Architecture of the Vibrio cholerae toxin-coregulated pilus machine revealed by electron cryotomography

Chang Y-W, Kjaer A, Ortega DR, Kovacikova G, Sutherland JA, Rettberg LA, Taylor RK, Jensen GJ




Architecture of the type IVa pilus machine

Chang Y-W, Rettberg LA, Treuner-Lange A, Iwasa J, Sogaard-Andersen L, Jensen GJ





Nature Methods

Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography

Chang YW, Chen S, Tocheva EI, Treuner-Lange A, Löbach S, Søgaard-Andersen L, Jensen GJ


Marine tubeworm metamorphosis induced by arrays of bacterial phage tail-like structures

Shikuma NJ*, Pilhofer M*, Weiss GL, Hadfield MG+, Jensen GJ+, Newman DK+



Nature Communications

Discovery of chlamydial peptidoglycan reveals bacteria with murein sacculi but without FtsZ

Pilhofer M*, Aistleitner K*, Biboy J, Gray J, Kuru E, Hall E, Brun YV, VanNieuwenhze MS, Vollmer W, Horn M+, Jensen GJ+

Molecular Biology of the Cell

Electron cryotomography of ESCRT assemblies and dividing Sulfolobus cells suggests that spiraling filaments are involved in membrane scission

Dobro MJ, Samson RY, Yu Z, McCullough J, Ding HJ, Chong PL, Bell SD, Jensen GJ

Molecular Microbiology

Architecture and assembly of the Gram-positive cell wall

Beeby M, Gumbart JC, Roux B, Jensen GJ

Nature Structural and Molecular Biology

Architecture of the major component of the type III secretion system export apparatus

Abrusci P, Vergara-Irigaray M, Johnson S, Beeby MD, Hendrixson DR, Roversi P, Friede ME, Deane JE, Jensen GJ, Tang CM, Lea SM

Journal of Molecular Biology

Unclosed HIV-1 capsids suggest a curled sheet model of assembly

Yu Z*, Dobro MJ*, Woodward CL, Levandovsky A, Danielson CM, Sandrin V, Shi J, Aiken C, Zandi R, Hope TJ, Jensen GJ




General protein diffusion barriers create compartments within bacterial cells

Schlimpert S*, Klein EA*, Briegel A, Hughes V, Kahnt J, Bolte K, Maier UG, Brun YV, Jensen GJ, Gitai Z, Thanbichler M


Type VI secretion requires a dynamic contractile phage tail-like structure

Basler M*, Pilhofer M*, Henderson GP, Jensen GJ+, Mekalanos JJ+



PLoS Biology

Microtubules in bacteria: Ancient tubulins build a five-protofilament homolog of the eukaryotic cytoskeleton

Pilhofer M+, Ladinsky MS, McDowall AW, Petroni G, Jensen GJ+

Molecular Microbiology

Activated chemoreceptor arrays remain intact and hexagonally packed

Briegel A, Beeby M, Thanbichler M, Jensen GJ

Current Biology

Organization of the smallest eukaryotic spindle

Gan L, Ladinsky MS, Jensen GJ


Peptidoglycan remodeling and conversion of an inner membrane into an outer membrane during sporulation

Tocheva EI, Matson EG, Morris DM, Moussavi F, Leadbetter JR, Jensen GJ

The EMBO Journal

Structural diversity of bacterial flagellar motors

Chen S*, Beeby M*, Murphy GE, Leadbetter JR, Hendrixson DR, Briegel A, Li Z, Shi J, Tocheva EI, Müller A, Dobro MJ, Jensen GJ


Alternative mechanism for bacteriophage adsorption to the motile bacterium Caulobacter crescentus

Guerrero-Ferreira RC, Viollier PH, Ely B, Poindexter JS, Georgieva M, Jensen GJ, Wright ER

Biochemical and Biophysical Research Communications

Long helical filaments are not seen encircling cells in electron cryotomograms of rod-shaped bacteria

Swulius MT, Chen S, Jane Ding H, Li Z, Briegel A, Pilhofer M, Tocheva EI, Lybarger SR, Johnson TL, Sandkvist M, Jensen GJ



Nature Cell Biology

The metabolic enzyme CTP synthase forms cytoskeletal filaments

Ingerson-Mahar M, Briegel A, Werner JN, Jensen GJ, Gitai Z

Journal of Molecular Biology

Organization, structure, and assembly of alpha-carboxysomes determined by electron cryotomography of intact cells

Iancu CV*, Morris DM*, Dou Z, Heinhorst S, Cannon GC, Jensen GJ



Microscopy and Microanalysis

An improved cryogen for plunge freezing

Tivol WF+, Briegel A, Jensen GJ

Molecular Microbiology

Novel ultrastructures of Treponema primitia and their implications for motility

Murphy GE, Matson EG, Leadbetter JR, Berg HC, Jensen GJ



The EMBO Journal

Electron cryotomography of immature HIV-1 virions reveals the structure of the CA and SP1 Gag shells

Wright ER*, Schooler JB*, Ding HJ, Kieffer C, Fillmore C, Sundquist WI, Jensen GJ





Journal of Molecular Biology

Three-dimensional structure of HIV-1 virus-like particles by electron cryotomography

Benjamin J, Ganser-Pornillos BK, Tivol WF, Sundquist WI, Jensen GJ