Publications – Microbial Cell Biology



Journal of Microbiology & Biology Education

The Atlas of Bacterial & Archaeal Cell Structure: an interactive open-access microbiology textbook

Oikonomou CM, Jensen GJ


A novel widespread bacterial structure related to the flagellar type III secretion system

Kaplan M, Oikonomou CM, Wood CR, Chreifi G, Ghosal D, Dobro MJ, Yao Q, McDowall A, Briegel A, Beeby M, Chang Y-W, Shaffer CL, Jensen GJ


In situ imaging of bacterial membrane projections and associated protein complexes using electron cryo-tomography

Kaplan M, Chreifi G, Metskas LA, Liedtke J, Wood CR, Oikonomou CM, Nicolas WJ, Subramanian P, Zacharoff LA, Wang Y, Chang Y-W, Beeby M, Dobro M, Zhu Y, McBride MJ, Briegel A, Shaffer C, Jensen GJ


Loss of the bacterial flagellar motor switch complex upon cell lysis

Kaplan M, Tocheva EI, Briegel A, Dobro MJ, Chang Y-W, Subramanian P, McDowall AW, Beeby M, Jensen GJ

Journal of Molecular Biology

Programmed flagellar ejection in Caulobacter crescentus leaves PL-subcomplexes

Kaplan M, Wang Y, Chreifi G, Zhang L, Chang Y-W, Jensen GJ

Protein Science

Measuring gas vesicle dimensions by electron microscopy

Dutka P, Malounda D, Metskas LA, Chen S, Hurt RC, Lu GJ, Jensen GJ+, Shapiro MK+



Journal of Bacteriology

Simulations of proposed mechanisms of FtsZ-driven cell constriction

Nguyen LT, Oikonomou CM, Jensen GJ

Journal of Bacteriology

Structure of the bacterial cellulose ribbon and its assembly-guiding cytoskeleton by electron cryotomography

Nicolas WJ, Ghosal D, Tocheva EI, Meyerowitz EM, Jensen GJ

Nature Communications

CryoEM structure of the Type IVa pilus secretin required for natural competence in Vibrio cholerae

Weaver SJ, Ortega DR, Sazinsky MH, Dalia TN, Dalia AB, Jensen GJ

Nature Communications

PilY1 and minor pilins form a complex priming the type IVa pilus in Myxococcus xanthus

Treuner-Lange A, Chang Y-W, Glatter T, Herfurth M, Lindow S, Chreifi G, Jensen GJ, Søgaard-Andersen L

Photodiagnosis and Photodynamic Therapy

Effects of antimicrobial photodynamic therapy on antibiotic-resistant Escherichia coli

Garcez AS*+, Kaplan M*, Jensen GJ, Scheidt FR, Oliveira EM, Suzuki SS


In situ structure and organisation of the type IX secretion system

Gorasia DG*, Chreifi G*, Seers CA, Butler CA, Heath JE, Glew MD, McBride MJ, Subramanian P, Kjaer A, Jensen GJ+, Veith PD+, Reynolds EC+

Nature Communications

Repurposing a chemosensory macromolecular machine

Ortega DR, Yang W, Subramanian P, Mann P, Kjær A, Chen S, Watts KJ, Pirbadian A, Collins DA, Kooger R, Kalyuzhnaya MG, Ringgaard S, Briegel A+, Jensen GJ+


Bacterial flagellar motor PL-ring disassembly subcomplexes are widespread and ancient

Kaplan M, Sweredoski MJ, Rodrigues JPGLM, Tocheva EI, Chang Y-W, Ortega DR, Beeby M, Jensen GJ


A prokaryotic membrane sculpting BAR domain protein

Phillips DA*, Zacharoff LA*, Hampton CM, Chong GW, Malanoski AP, Metskas LA, Xu S, Bird LJ, Eddie BJ, Jensen GJ, Drummy LF, El-Naggar MY, Glaven SM



Nature Microbiology

In vivo structure of the Legionella type II secretion system by electron cryotomography

Ghosal D, Kim KW, Zheng H, Kaplan M, Truchan HK, Lopez AE, McIntire IE, Vogel JP, Cianciotto NP, Jensen GJ


Distinct chemotaxis protein paralogs assemble into chemoreceptor signaling arrays to coordinate signaling output

O’Neal L*, Gullett JM*, Aksenova A, Hubler A, Briegel A, Ortega D, Kjaer A, Jensen G, Alexandre G

EMBO Journal

In situ imaging of the bacterial flagellar motor disassembly and assembly processes

Kaplan M, Subramanian P, Ghosal D, Oikonomou CM, Pirbadian S, Starwalt-Lee R, Mageswaran SK, Ortega DR, Gralnick JA, El-Naggar MY, Jensen GJ

Nature Microbiology

Molecular architecture, polar targeting and biogenesis of the Legionella Dot/Icm T4SS

Ghosal D*, Jeong KC*, Chang Y-W, Gyore J, Teng L, Gardner A, Vogel JP+, Jensen GJ+

Microbiology Spectrum

Electron cryotomography of bacterial secretion systems

Oikonomou CM, Jensen GJ

Nature Communications

Simulations suggest a constrictive force is required for Gram-negative bacterial cell division

Nguyen LT, Oikonomou CM, Ding HJ, Kaplan M, Yao Q, Chang Y-W, Beeby M, Jensen GJ


The presence and absence of periplasmic rings in bacterial flagellar motors correlates with stator type

Kaplan M, Ghosal D, Subramanian P, Oikonomou CM, Kjaer A, Pirbadian S, Ortega DR, Briegel A, El-Naggar MY, Jensen GJ




Programmed secretion arrest and receptor-triggered toxin export during antibacterial contact-dependent growth inhibition

Ruhe ZC*, Subramanian P*, Song K*, Nguyen JY, Stevens TA, Low DA, Jensen GJ+, Hayes CS+

Journal of Physical Chemistry B

Selective permeability of carboxysome shell pores to anionic molecules

Mahinthichaichan P*, Morris DM*, Wang Y, Jensen GJ+, Tajkhorshid E+

ISME Journal

Nutrient transport suggests an evolutionary basis for charged archaeal surface layer proteins

Li P-N, Herrmann J, Tolar BB, Poitevin F, Ramdasi R, Bargar JR, Stahl DA, Jensen GJ, Francis CA+, Wakatsuki S+, van den Bedem H+

Cell Reports

In vivo structures of the Helicobacter pylori cag type IV secretion system

Chang Y-W*, Shaffer CL*, Rettberg LA, Ghosal D, Jensen GJ


Ultrastructure of Shewanella oneidensis MR-1 nanowires revealed by electron cryotomography

Subramanian P*, Pirbadian S*, El-Naggar MY+, Jensen GJ+

Molecular Microbiology

Electron cryotomography of Mycoplasma pneumoniae mutants correlates terminal organelle architectural features and function

Krause DC+, Chen S, Shi J, Jensen A, Sheppard ES, Jensen GJ




Assigning chemoreceptors to chemosensory pathways in Pseudomonas aeruginosa

Ortega DR*, Fleetwood AD*, Krell T, Harwood CS, Jensen GJ, Zhulin IB

International Journal of Systematic & Evolutionary Microbiology

Nitrosopumilus maritimus gen. nov., sp. nov., Nitrosopumilus cobalaminigenes sp. nov., Nitrosopumilus oxyclinae sp. nov., and Nitrosopumilus ureiphilus sp. nov., four marine ammonia-oxidizing archaea of the phylum Thaumarchaeota

Qin W, Heal KR, Ramdasi R, Kobelt JN, Martens-Habbena W, Bertagnolli AD, Amin SA, Walker CB, Urakawa H, Könneke M, Devol AH, Moffett JW, Armbrust EV, Jensen GJ, Ingalls AE, Stahl DA

Molecular Microbiology

LytM factors affect the recruitment of autolysins to the cell division site in Caulobacter crescentus

Zielińska A*, Billini M*, Möll A, Kremer K, Briegel A, Martinez AI, Jensen GJ, Thanbichler M

EMBO Reports

Morphology of the archaellar motor and associated cytoplasmic cone in Thermococcus kodakaraensis

Briegel A, Oikonomou CM, Chang Y-W, Kjaer A, Huang AN, Kim KW, Ghosal D, Nguyen HH, Kenny D, Ogorzalek Loo RR, Gunsalus RP, Jensen GJ

Journal of Bacteriology

Uncharacterized bacterial structures revealed by electron cryotomography

Dobro MJ, Oikonomou CM, Piper A, Cohen J, Guo K, Jensen T, Tadayon J, Donermeyer J, Park Y, Solis BA, Kjaer A, Jewett AI, McDowall AW, Chen S, Chang Y-W, Shi J, Subramanian P, Iancu CV, Li Z, Briegel A, Tocheva EI, Pilhofer M, Jensen GJ

Subcellular Biochemistry

The Structure, Function and Roles of the Archaeal ESCRT Apparatus

Samson RY, Dobro MJ, Jensen GJ, Bell SD

The EMBO Journal

Short FtsZ filaments can drive asymmetric cell envelope constriction at the onset of bacterial cytokinesis

Yao Q, Jewett AI, Chang Y-W, Oikonomou CM, Beeby M, Iancu CV, Briegel A, Ghosal D, Jensen GJ


Giant viruses with an expanded complement of translation system components

Schulz F+, Yutin N, Ivanova NN, Ortega DR, Lee TK, Vierheilig J, Daims H, Horn M, Wagner M, Jensen GJ, Kyrpides NC, Koonin EV+, Woyke T+

EMBO Reports

Structure of the Legionella Dot/Icm type IV secretion system in situ by electron cryotomography

Ghosal D, Chang Y-W, Jeong KC, Vogel JP, Jensen GJ

Nature Microbiology

Architecture of the Vibrio cholerae toxin-coregulated pilus machine revealed by electron cryotomography

Chang Y-W, Kjaer A, Ortega DR, Kovacikova G, Sutherland JA, Rettberg LA, Taylor RK, Jensen GJ



Molecular Microbiology

Dynamics of the peptidoglycan biosynthetic machinery in the stalked budding bacterium Hyphomonas neptunium

Cserti E, Rosskopf S, Chang Y-W, Eisheuer S, Selter L, Shi J, Regh C, Koert U, Jensen GJ, Thanbichler M

Molecular Microbiology

Starvation and recovery in the deep-sea methanotroph Methyloprofundus sedimenti

Tavormina PL+, Kellermann MY, Antony CP, Tocheva E, Dalleska N, Jensen AJ, Valentine DL, Hinrichs KU, Jensen G, Dubilier N, Orphan VJ


Diverse high-torque bacterial flagellar motors assemble wider stator rings using a conserved protein scaffold

Beeby M, Ribardo DA, Brennan CA, Ruby EG, Jensen GJ, Hendrixson DR


Architecture of the type IVa pilus machine

Chang Y-W, Rettberg LA, Treuner-Lange A, Iwasa J, Sogaard-Andersen L, Jensen GJ

Nature Reviews Microbiology

A new view into prokaryotic cell biology from electron cryotomography

Oikonomou CM, Chang Y-W, Jensen GJ



Environmental Microbiology Reports

Structural conservation of chemotaxis machinery across Archaea and Bacteria

Briegel A, Ortega DR, Huang AN, Oikonomou CM, Gunsalus RP, Jensen GJ

Journal of Biomolecular Screening

Streptomyces: a screening tool for bacterial cell division inhibitors

Jani C, Tocheva EI, McAuley S, Craney A, Jensen GJ, Nodwell J



Microbiology Open

Ultrastructure and complex polar architecture of the human pathogen Campylobacter jejuni

Müller A, Beeby M, McDowall AW, Chow J, Jensen GJ, Clemons WM Jr.

Nature Methods

Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography

Chang YW, Chen S, Tocheva EI, Treuner-Lange A, Löbach S, Søgaard-Andersen L, Jensen GJ


Structure of bacterial cytoplasmic chemoreceptor arrays and implications for chemotactic signaling

Briegel A, Ladinsky MS, Oikonomou C, Jones CW, Harris MJ, Fowler DJ, Chang YW, Thompson LK, Armitage JP, Jensen GJ

PLOS Computational Biology

Escherichia coli peptidoglycan structure and mechanics as predicted by atomic-scale simulations

Gumbart JC+, Beeby M, Jensen GJ, Roux B+


New insights into bacterial chemoreceptor array structure and assembly from electron cryotomography

Briegel A, Wong ML, Hodges HL, Oikonomou CM, Piasta KN, Harris MJ, Fowler DJ, Thompson LK, Falke JJ, Kiessling LL, Jensen GJ

Journal of Bacteriology

Structure and expression of propanediol utilization microcompartments in Acetonema longum

Tocheva EI, Matson EG, Cheng SN, Chen WG, Leadbetter JR, Jensen GJ


Marine tubeworm metamorphosis induced by arrays of bacterial phage tail-like structures

Shikuma NJ*, Pilhofer M*, Weiss GL, Hadfield MG+, Jensen GJ+, Newman DK+



Nature Communications

Discovery of chlamydial peptidoglycan reveals bacteria with murein sacculi but without FtsZ

Pilhofer M*, Aistleitner K*, Biboy J, Gray J, Kuru E, Hall E, Brun YV, VanNieuwenhze MS, Vollmer W, Horn M+, Jensen GJ+

Environmental Microbiology

Architecture and host interface of environmental chlamydiae revealed by electron cryotomography

Pilhofer M*, Aistleitner K*, Ladinsky MS, König L, Horn M+, Jensen GJ+

Journal of Bacteriology

Polyphosphate storage during sporulation in the gram-negative bacterium Acetonema longum

Tocheva EI, Dekas AE, McGlynn SE, Morris D, Orphan VJ, Jensen GJ

Molecular Microbiology

The mobility of two kinase domains in the Escherichia coli chemoreceptor array varies with signalling state

Briegel A, Ames P, Gumbart JC, Oikonomou CM, Parkinson JS, Jensen GJ

Molecular Biology of the Cell

Electron cryotomography of ESCRT assemblies and dividing Sulfolobus cells suggests that spiraling filaments are involved in membrane scission

Dobro MJ, Samson RY, Yu Z, McCullough J, Ding HJ, Chong PL, Bell SD, Jensen GJ

Molecular Microbiology

Architecture and assembly of the Gram-positive cell wall

Beeby M, Gumbart JC, Roux B, Jensen GJ

Molecular Microbiology

Peptidoglycan transformations during Bacillus subtilis sporulation

Tocheva EI, López-Garrido J, Hughes HV, Fredlund J, Kuru E, Vannieuwenhze MS, Brun YV, Pogliano K, Jensen GJ

Nature Structural and Molecular Biology

Architecture of the major component of the type III secretion system export apparatus

Abrusci P, Vergara-Irigaray M, Johnson S, Beeby MD, Hendrixson DR, Roversi P, Friede ME, Deane JE, Jensen GJ, Tang CM, Lea SM




General protein diffusion barriers create compartments within bacterial cells

Schlimpert S*, Klein EA*, Briegel A, Hughes V, Kahnt J, Bolte K, Maier UG, Brun YV, Jensen GJ, Gitai Z, Thanbichler M

Current Opinion in Cell Biology

The bacterial cytoskeleton: more than twisted filaments

Pilhofer M, Jensen GJ

Genes and Development

A multidomain hub anchors the chromosome segregation and chemotactic machinery to the bacterial pole

Yamaichi Y*, Bruckner R*, Ringgaard S, Möll A, Cameron DE, Briegel A, Jensen GJ, Davis BM, Waldor MK


Type VI secretion requires a dynamic contractile phage tail-like structure

Basler M*, Pilhofer M*, Henderson GP, Jensen GJ+, Mekalanos JJ+

Journal of Bacteriology

Growth and localization of polyhydroxybutyrate granules in Ralstonia eutropha

Beeby M, Cho M, Stubbe J, Jensen GJ

Quarterly Reviews of Biophysics

Electron Tomography of Cells

Gan L, Jensen GJ



PLoS Biology

Microtubules in bacteria: Ancient tubulins build a five-protofilament homolog of the eukaryotic cytoskeleton

Pilhofer M+, Ladinsky MS, McDowall AW, Petroni G, Jensen GJ+

Molecular Microbiology

Activated chemoreceptor arrays remain intact and hexagonally packed

Briegel A, Beeby M, Thanbichler M, Jensen GJ


Peptidoglycan remodeling and conversion of an inner membrane into an outer membrane during sporulation

Tocheva EI, Matson EG, Morris DM, Moussavi F, Leadbetter JR, Jensen GJ

PLoS One

Nanopods: a new bacterial structure and mechanism for deployment of outer membrane vesicles

Shetty A, Chen S, Tocheva EI, Jensen GJ, Hickey WJ

The EMBO Journal

Structural diversity of bacterial flagellar motors

Chen S*, Beeby M*, Murphy GE, Leadbetter JR, Hendrixson DR, Briegel A, Li Z, Shi J, Tocheva EI, Müller A, Dobro MJ, Jensen GJ


Alternative mechanism for bacteriophage adsorption to the motile bacterium Caulobacter crescentus

Guerrero-Ferreira RC, Viollier PH, Ely B, Poindexter JS, Georgieva M, Jensen GJ, Wright ER

Biochemical and Biophysical Research Communications

Long helical filaments are not seen encircling cells in electron cryotomograms of rod-shaped bacteria

Swulius MT, Chen S, Jane Ding H, Li Z, Briegel A, Pilhofer M, Tocheva EI, Lybarger SR, Johnson TL, Sandkvist M, Jensen GJ




In vivo assembly of an archaeal virus studied with whole-cell electron cryotomography

Fu CY, Wang K, Gan L, Lanman J, Khayat R, Young MJ, Jensen GJ, Doerschuk PC, Johnson JE

Methods in Cell Biology

Bacterial TEM: new insights from cryo-microscopy

Pilhofer M, Ladinsky MS, McDowall AW, Jensen GJ

Nature Cell Biology

The metabolic enzyme CTP synthase forms cytoskeletal filaments

Ingerson-Mahar M, Briegel A, Werner JN, Jensen GJ, Gitai Z

Cold Spring Harbor Perspectives in Biology

Electron cryotomography

Tocheva EI, Li Z, Jensen GJ

Molecular Microbiology

DipM, a new factor required for peptidoglycan remodelling during cell division in Caulobacter crescentus

Möll A, Schlimpert S, Briegel A, Jensen GJ, Thanbichler M


Electron cryotomography of bacterial cells

Chen S, McDowall A, Dobro MJ, Briegel A, Ladinsky M, Shi J, Tocheva EI, Beeby M, Pilhofer M, Ding HJ, Li Z, Gan L, Morris DM, Jensen GJ

Journal of Bacteriology

Mutations in the Lipopolysaccharide biosynthesis pathway interfere with crescentin-mediated cell curvature in Caulobacter crescentus

Cabeen MT*, Murolo MA*, Briegel A, Bui NK, Vollmer W, Ausmees N, Jensen GJ, Jacobs-Wagner C

The EMBO Journal

Bactofilins, a ubiquitous class of cytoskeletal proteins mediating polar localization of a cell wall synthase in Caulobacter crescentus

Kühn J, Briegel A, Mörschel E, Kahnt J, Leser K, Wick S, Jensen GJ, Thanbichler M

Journal of Molecular Biology

Organization, structure, and assembly of alpha-carboxysomes determined by electron cryotomography of intact cells

Iancu CV*, Morris DM*, Dou Z, Heinhorst S, Cannon GC, Jensen GJ




Universal architecture of bacterial chemoreceptor arrays

Briegel A, Ortega DR, Tocheva EI, Wuichet K, Li Z, Chen S, Müller A, Iancu CV, Murphy GE, Dobro MJ, Zhulin IB, Jensen GJ

Current Opinion in Microbiology

Electron cryotomography: a new view into microbial ultrastructure

Li Z, Jensen GJ


Cell biology. Protein filaments caught in the act

Jensen GJ

Comment on

Electron cryomicroscopy of E. coli reveals filament bundles involved in plasmid DNA segregation



Molecular Microbiology

Location and architecture of the Caulobacter crescentus chemoreceptor array

Briegel A, Ding HJ, Li Z, Werner J, Gitai Z, Dias DP, Jensen RB, Jensen GJ

Annual Review of Biochemistry

Toward a biomechanical understanding of whole bacterial cells

Morris DM, Jensen GJ

Molecular Microbiology

Novel ultrastructures of Treponema primitia and their implications for motility

Murphy GE, Matson EG, Leadbetter JR, Berg HC, Jensen GJ



International Journal of Systematic and Evolutionary Microbiology

Tetrasphaera remsis sp. nov., isolated from the Regenerative Enclosed Life Support Module Simulator (REMS) air system

Osman S, Moissl C, Hosoya N, Briegel A, Mayliraj S, Satomi M, Venkateswaran K


Electron cryotomography

Murphy GE, Jensen GJ

Journal of Molecular Biology

The structure of isolated Synechococcus strain WH8102 carboxysomes as revealed by electron cryotomography

Iancu CV, Ding HJ, Morris DM, Dias DP, Gonzales AD, Martino A, Jensen GJ

Current Opinion in Structural Biology

How electron cryotomography is opening a new window onto prokaryotic ultrastructure

Jensen GJ, Briegel A

Int’l Journal of Systematic & Evolutionary Microbiology

Haloquadratum walsbyi gen. nov., sp. nov., the square haloarchaeon of Walsby, isolated from saltern crystallizers in Australia and Spain

Burns DG, Janssen PH, Itoh T, Kamekura M, Li Z, Jensen G, Rodriguez-Valera F, Bolhuis H, Dyall-Smith ML



Molecular Microbiology

Multiple large filament bundles observed in Caulobacter crescentus by electron cryotomography

Briegel A, Dias DP, Li Z, Jensen RB, Frangakis AS, Jensen GJ