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eLife
A cryogenic, coincident fluorescence, electron and ion beam microscope
Boltje DB, Hoogenboom JP, Jakobi AJ, Jensen GJ, Jonker CTH, Kaag MJ, Koster AJ, Last MGF, de Agrela Pinto C, Plitzko JM, Raunser S, Tacke S, Wang Z, van der Wee EB, Wepf R, den Hoedt S
Journal of Structural Biology: X
Subtomogram averaging for biophysical analysis and supramolecular context
Metskas LA, Wilfong R, Jensen GJ
bioRxiv
Cryo-EM structure of the Agrobacterium tumefaciens type IV secretion system-associated T-pilus reveals stoichiometric protein-phospholipid assembly
Kreida S*, Narita A*, Johnson MD, Tocheva EI, Das A, Ghosal D+, Jensen GJ+
Biophysical Journal
Geometric effects in gas vesicle buckling under ultrasound
Salahshoor H*, Yao Y*, Dutka P*, Nyström NN, Jin Z, Min E, Malounda D, Jensen GJ, Ortiz M, Shapiro MG
bioRxiv
Structural remodeling of Coxiella burnetii during its biphasic developmental cycle revealed by cryo-electron tomography
Kaplan M*, Shepherd DC*, Vankadari N, Kim KW, Larson CL, Dutka P, Beare PA, Krzymowski E, Heinzen RA, Jensen GJ+, Ghosal D+
Nature Communications
Rubisco forms a lattice inside alpha-carboxysomes
Metskas LA, Ortega D, Oltrogge LM, Blikstad C, Lovejoy DR, Laughlin TG, Savage DF, Jensen GJ
Nature
Archaic chaperone-usher pili self-secrete into superelastic zigzag springs architecture
Pakharukova N*, Malmi H*, Tuittila M, Dahlberg T, Ghosal D, Chang Y-W, Myint SL, Paavilainen S, Knight SD, Lamminmäki U, Uhlin BE, Andersson M, Jensen G, Zavialov AV
Journal of Bacteriology
Discovery of a novel inner membrane-associated bacterial structure related to the flagellar type III secretion system
Kaplan M, Oikonomou CM, Wood CR, Chreifi G, Ghosal D, Dobro MJ, Yao Q, Pal RR, Baidya AK, Liu Y, Maggi S, McDowall AW, Ben-Yehuda S, Rosenshine I, Briegel A, Beeby M, Chang Y-W, Shaffer CL, Jensen GJ
bioRxiv
Structure of Anabaena flos-aquae gas vesicles revealed by cryo-ET
Dutka P, Metskas LA, Hurt RC, Salahshoor H, Wang T-Y, Malounda D, Lu G, Chou T-F, Shapiro MG+, Jensen GJ+
bioRxiv
Dynamic structural adaptations enable the endobiotic predation of Bdellovibrio bacteriovorus
Kaplan M*, Chang Y-W*, Oikonomou CM, Nicolas WJ, Jewett AI, Kreida S, Dutka P, Rettberg LA, Maggi S, Jensen GJ
Current Biology
Cryo-electron tomography of the onion cell wall shows bimodally oriented cellulose fibers and reticulated homogalacturonan networks
Nicolas WJ, Faessler F, Dutka P, Schur FKM, Jensen GJ, Meyerowitz EM
Journal of Structural Biology
Montage electron tomography of vitrified specimens
Peck A, Carter SD, Mai H, Chen S, Burt A, Jensen GJ
EMBO Journal
Novel transient cytoplasmic rings stabilize assembling bacterial flagellar motors
Kaplan M, Oikonomou CM, Wood CR, Chreifi G, Subramanian P, Ortega DR, Chang Y-W, Beeby M, Shaffer CL, Jensen GJ
bioRxiv
In situ architecture of human kinetochore-microtubule interface visualized by cryo-electron tomography
Zhao W, Jensen GJ
Communications Biology
UVC inactivation of pathogenic samples suitable for cryo-EM analysis
Depelteau JS, Renault L, Althof N, Cassidy CK, Mendonça LM, Jensen GJ, Resch GP, Briegel A
bioRxiv
Nanoscale details of mitochondrial fission revealed by cryo-electron tomography
Mageswaran SK*, Grotjahn DA*+, Zeng X, Barad BA, Medina M, Hoang MH, Dobro MJ, Chang Y-W, Xu M, Yang WY, Jensen GJ+
eLife
A bacterial membrane sculpting protein with BAR domain-like activity
Phillips DA*, Zacharoff LA*, Hampton CM, Chong GW, Malanoski AP, Metskas LA, Xu S, Bird LJ, Eddie BJ, Miklos AE, Jensen GJ, Drummy LF, El-Naggar MY, Glaven SM
Science
The stress-sensing domain of activated IRE1α forms helical filaments in narrow ER membrane tubes
Tran N-H*, Carter SD*, De Maziére A, Ashkenazi A, Klumperman J, Walter P+, Jensen GJ+
Journal of Microbiology & Biology Education
The Atlas of Bacterial & Archaeal Cell Structure: an interactive open-access microbiology textbook
Oikonomou CM, Jensen GJ
eLife
In situ imaging of bacterial membrane projections and associated protein complexes using electron cryo-tomography
Kaplan M, Chreifi G, Metskas LA, Liedtke J, Wood CR, Oikonomou CM, Nicolas WJ, Subramanian P, Zacharoff LA, Wang Y, Chang Y-W, Beeby M, Dobro M, Zhu Y, McBride MJ, Briegel A, Shaffer C, Jensen GJ
mBio
Loss of the bacterial flagellar motor switch complex upon cell lysis
Kaplan M, Tocheva EI, Briegel A, Dobro MJ, Chang Y-W, Subramanian P, McDowall AW, Beeby M, Jensen GJ
PNAS
Generation of ordered protein assemblies using rigid three-body fusion
Vulovic I, Yao Q*, Park Y-J*, Courbet A, Norris A, Busch F, Sahasrabuddhe A, Merten H, Sahtoe DD, Ueda G, Fallas JA, Weaver SJ, Hsia Y, Langan RA, Plückthun A, Wysocki VH, Veesler D, Jensen GJ, Baker D
Acta Crystallographica Section D
Challenges in solving structures from radiation-damaged tomograms of protein nanocrystals assessed by simulation
Peck A, Yao Q, Brewster AS, Zwart PH, Heumann JM, Sauter NK, Jensen GJ
Journal of Molecular Biology
Programmed flagellar ejection in Caulobacter crescentus leaves PL-subcomplexes
Kaplan M, Wang Y, Chreifi G, Zhang L, Chang Y-W, Jensen GJ
Science Advances
A cryo-electron tomography workflow reveals protrusion-mediated shedding on injured plasma membrane
Mageswaran SK, Yang WY+, Chakrabarty Y, Oikonomou CM, Jensen GJ+
Journal of Structural Biology
Rapid tilt-series method for cryo-electron tomography: Characterizing stage behavior during FISE acquisition
Chreifi G, Chen S, Jensen GJ
Protein Science
Measuring gas vesicle dimensions by electron microscopy
Dutka P, Malounda D, Metskas LA, Chen S, Hurt RC, Lu GJ, Jensen GJ+, Shapiro MK+
Journal of Molecular Biology
Moltemplate: a tool for coarse-grained modeling of complex biological matter and soft condensed matter physics
Jewett AI+, Stelter D, Lambert J, Saladi SM, Roscioni OM, Ricci M, Autin L, Maritan M, Bashusqeh SM, Keyes T, Dame RT, Shea J-E, Jensen GJ, Goodsell DS+
Methods in Molecular Biology: CryoEM
In situ imaging and structure determination of biomolecular complexes using electron cryo-tomography
Kaplan M, Nicolas WJ, Zhao W, Carter SD, Metskas LA, Chreifi G, Ghosal D, Jensen GJ
Science Advances
Visualizing insulin vesicle neighborhoods in β cells by cryo-electron tomography
Zhang X, Carter SD, Singla J, White KL, Butler PC, Stevens RC+, Jensen GJ+
Journal of Bacteriology
Simulations of proposed mechanisms of FtsZ-driven cell constriction
Nguyen LT, Oikonomou CM, Jensen GJ
Journal of Bacteriology
Structure of the bacterial cellulose ribbon and its assembly-guiding cytoskeleton by electron cryotomography
Nicolas WJ, Ghosal D, Tocheva EI, Meyerowitz EM, Jensen GJ
PNAS
Correlated cryogenic fluorescence microscopy and electron cryo-tomography shows that exogenous TRIM5α can form hexagonal lattices or autophagy aggregates in vivo
Carter SD, Mamede JI, Hope TJ, Jensen GJ
Nature Communications
CryoEM structure of the Type IVa pilus secretin required for natural competence in Vibrio cholerae
Weaver SJ, Ortega DR, Sazinsky MH, Dalia TN, Dalia AB, Jensen GJ
Nature Communications
PilY1 and minor pilins form a complex priming the type IVa pilus in Myxococcus xanthus
Treuner-Lange A, Chang Y-W, Glatter T, Herfurth M, Lindow S, Chreifi G, Jensen GJ, Søgaard-Andersen L
Photodiagnosis and Photodynamic Therapy
Effects of antimicrobial photodynamic therapy on antibiotic-resistant Escherichia coli
Garcez AS*+, Kaplan M*, Jensen GJ, Scheidt FR, Oliveira EM, Suzuki SS
bioRxiv
In situ structure and organisation of the type IX secretion system
Gorasia DG*, Chreifi G*, Seers CA, Butler CA, Heath JE, Glew MD, McBride MJ, Subramanian P, Kjaer A, Jensen GJ+, Veith PD+, Reynolds EC+
Nature Communications
Repurposing a chemosensory macromolecular machine
Ortega DR, Yang W, Subramanian P, Mann P, Kjær A, Chen S, Watts KJ, Pirbadian A, Collins DA, Kooger R, Kalyuzhnaya MG, Ringgaard S, Briegel A+, Jensen GJ+
PNAS
Bacterial flagellar motor PL-ring disassembly subcomplexes are widespread and ancient
Kaplan M, Sweredoski MJ, Rodrigues JPGLM, Tocheva EI, Chang Y-W, Ortega DR, Beeby M, Jensen GJ
Science Advances
Ribosome-associated vesicles: A dynamic subcompartment of the endoplasmic reticulum in secretory cells
Carter SD*, Hampton CM*, Langlois R*, Melero R, Farino ZJ, Calderon MJ, Li W, Wallace CT, Tran NH, Grassucci RA, Siegmund SE, Pemberton J, Morgenstern TJ, Eisenman L, Aguilar JI, Greenberg NL, Levy ES, Yi E, Mitchell WG, Rice WJ, Wigge C, Pilli J, George EW, Aslanoglou D, Courel M, Freyberg RJ, Javitch JA, Wills ZP, Area-Gomez E, Shiva S, Bartolini F, Volchuk A, Murray SA, Aridor M, Fish KN, Walter P, Balla T, Fass D, Wolf SG, Watkins SC, Carazo JM, Jensen GJ+, Frank J+, Freyberg Z+
Nature Microbiology
In vivo structure of the Legionella type II secretion system by electron cryotomography
Ghosal D, Kim KW, Zheng H, Kaplan M, Truchan HK, Lopez AE, McIntire IE, Vogel JP, Cianciotto NP, Jensen GJ
mBio
Bacterial swarming reduces Proteus mirabilis and Vibrio parahaemolyticus cell stiffness and increases β-lactam susceptibility
Auer GK, Oliver PM, Rajendram M, Lin T-Y, Yao Q, Jensen GJ, Weibel DB
Gateways 2019 (Conference)
DDX-Interface: A factory of interoperable scientific gateways
James A, Chacon R, Buddenhagen J, Ortega DR, Rees-Lee P, Jensen G, James DR
mBio
Distinct chemotaxis protein paralogs assemble into chemoreceptor signaling arrays to coordinate signaling output
O’Neal L*, Gullett JM*, Aksenova A, Hubler A, Briegel A, Ortega D, Kjaer A, Jensen G, Alexandre G
Structure
Qualitative analyses of polishing and precoating FIB milled crystals for MicroED
Martynowycz MW, Zhao W, Hattne J, Jensen GJ, Gonen T
bioRxiv
Regular Architecture (RegArch): A standard expression language for describing protein architectures
Ortega DR, Jensen GJ
EMBO Journal
In situ imaging of the bacterial flagellar motor disassembly and assembly processes
Kaplan M, Subramanian P, Ghosal D, Oikonomou CM, Pirbadian S, Starwalt-Lee R, Mageswaran SK, Ortega DR, Gralnick JA, El-Naggar MY, Jensen GJ
Structure
Fusion of DARPin to aldolase enables visualization of small protein by cryoEM
Yao Q*, Weaver SJ*, Mock J-Y, Jensen GJ
Nature Microbiology
Molecular architecture, polar targeting and biogenesis of the Legionella Dot/Icm T4SS
Ghosal D*, Jeong KC*, Chang Y-W, Gyore J, Teng L, Gardner A, Vogel JP+, Jensen GJ+
PLOS One
ETDB-Caltech: a blockchain-based distributed public database for electron tomography
Ortega DR, Oikonomou CM, Ding HJ, Rees-Lee P, Alexandria, Jensen GJ
Microbiology Spectrum
Electron cryotomography of bacterial secretion systems
Oikonomou CM, Jensen GJ
Nature Communications
Simulations suggest a constrictive force is required for Gram-negative bacterial cell division
Nguyen LT, Oikonomou CM, Ding HJ, Kaplan M, Yao Q, Chang Y-W, Beeby M, Jensen GJ
Structure
De novo structural pattern mining in cellular electron cryotomograms
Xu M, Singla J, Tocheva EI, Chang Y-W, Stevens RC, Jensen GJ, Alber F
Methods in Molecular Biology
In situ imaging and structure determination of bacterial toxin delivery systems using electron cryotomography
Ghosal D*, Kaplan M*, Chang Y-W, Jensen GJ
eLife
FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles
Azubel M, Carter SD, Weiszmann J, Zhang J, Jensen GJ, Li Y, Kornberg RD
Structure
Collection of continuous rotation MicroED data from ion beam-milled crystals of any size
Martynowycz MW, Zhao W, Hattne J, Jensen GJ, Gonen T
eLife
The presence and absence of periplasmic rings in bacterial flagellar motors correlates with stator type
Kaplan M, Ghosal D, Subramanian P, Oikonomou CM, Kjaer A, Pirbadian S, Ortega DR, Briegel A, El-Naggar MY, Jensen GJ
Journal of Structural Biology
Rapid tilt-series acquisition for electron cryotomography
Chreifi G*, Chen S*, Metskas LA, Kaplan M, Jensen GJ
Cell
Programmed secretion arrest and receptor-triggered toxin export during antibacterial contact-dependent growth inhibition
Ruhe ZC*, Subramanian P*, Song K*, Nguyen JY, Stevens TA, Low DA, Jensen GJ+, Hayes CS+
iScience
Three-dimensional analysis of mitochondrial crista ultrastructure in a patient with Leigh Syndrome by in situ cryoelectron tomography
Siegmund SE, Grassucci R, Carter SD, Barca E, Farino ZJ, Juanola-Falgarona M, Zhang P, Tanji K, Hirano M, Schon EA, Frank J, Freyberg Z
Journal of Physical Chemistry B
Selective permeability of carboxysome shell pores to anionic molecules
Mahinthichaichan P*, Morris DM*, Wang Y, Jensen GJ+, Tajkhorshid E+
ISME Journal
Nutrient transport suggests an evolutionary basis for charged archaeal surface layer proteins
Li P-N, Herrmann J, Tolar BB, Poitevin F, Ramdasi R, Bargar JR, Stahl DA, Jensen GJ, Francis CA+, Wakatsuki S+, van den Bedem H+
Molecular Biology of the Cell
Coarse-grained simulations of actomyosin rings point to a nodeless model involving both unipolar and bipolar myosins
Nguyen LT, Swulius MT, Aich S, Mishra M, Jensen GJ
Cell Reports
In vivo structures of the Helicobacter pylori cag type IV secretion system
Chang Y-W*, Shaffer CL*, Rettberg LA, Ghosal D, Jensen GJ
PNAS
Ultrastructure of Shewanella oneidensis MR-1 nanowires revealed by electron cryotomography
Subramanian P*, Pirbadian S*, El-Naggar MY+, Jensen GJ+
Molecular Microbiology
Electron cryotomography of Mycoplasma pneumoniae mutants correlates terminal organelle architectural features and function
Krause DC+, Chen S, Shi J, Jensen A, Sheppard ES, Jensen GJ
PNAS
Structure of the fission yeast actomyosin ring during constriction
Swulius MT*, Nguyen LT*, Ladinsky MS, Ortega DR, Aich S, Mishra M, Jensen GJ
Nature Microbiology
Comment: The development of cryo-EM and how it has advanced microbiology
Oikonomou CM, Jensen GJ
PNAS
Assigning chemoreceptors to chemosensory pathways in Pseudomonas aeruginosa
Ortega DR*, Fleetwood AD*, Krell T, Harwood CS, Jensen GJ, Zhulin IB
Journal of Structural Biology
Distinguishing signal from autofluorescence in cryogenic correlated light and electron microscopy of mammalian cells
Carter SD*, Mageswaran SK*, Farino ZJ, Mamede JI, Oikonomou CM, Hope TJ, Freyberg Z+, Jensen GJ+
International Journal of Systematic & Evolutionary Microbiology
Nitrosopumilus maritimus gen. nov., sp. nov., Nitrosopumilus cobalaminigenes sp. nov., Nitrosopumilus oxyclinae sp. nov., and Nitrosopumilus ureiphilus sp. nov., four marine ammonia-oxidizing archaea of the phylum Thaumarchaeota
Qin W, Heal KR, Ramdasi R, Kobelt JN, Martens-Habbena W, Bertagnolli AD, Amin SA, Walker CB, Urakawa H, Könneke M, Devol AH, Moffett JW, Armbrust EV, Jensen GJ, Ingalls AE, Stahl DA
PLoS Genetics
FtsEX-mediated regulation of the final stages of cell division reveals morphogenetic plasticity in Caulobacter crescentus
Meier EL, Daitch AK, Yao Q, Bhargava A, Jensen GJ, Goley ED
Molecular Microbiology
LytM factors affect the recruitment of autolysins to the cell division site in Caulobacter crescentus
Zielińska A*, Billini M*, Möll A, Kremer K, Briegel A, Martinez AI, Jensen GJ, Thanbichler M
Journal of Structural Biology
Best practices for managing large cryoEM facilities
Alewijnse B, Ashton AW, Chambers MG, Chen S, Cheng A, Ebrahim M, Eng E, Hagen WJH, Koster AJ, Lopez CS, Lukoyanova N, Ortega J, Renault L, Reyntjens S, Rice WJ, Scapin G, Schrijver R, Siebert A, Stagg SM, Grum-Tokars V, Wright ER, Wu S, Yu Z, Zhou ZH, Carragher B, Potter CS
Angewandte Chemie International Edition
Photon-induced near-field electron microscopy (PINEM) of eukaryotic cells
Kaplan M, Yoo BK, Tang J+, Karam T, Liao B, Majumdar B, Baltimore D, Jensen G+, Zewail A
EMBO Reports
Morphology of the archaellar motor and associated cytoplasmic cone in Thermococcus kodakaraensis
Briegel A, Oikonomou CM, Chang Y-W, Kjaer A, Huang AN, Kim KW, Ghosal D, Nguyen HH, Kenny D, Ogorzalek Loo RR, Gunsalus RP, Jensen GJ
PNAS
Polar delivery of Legionella type IV secretion system substrates is essential for virulence
Jeong KC, Ghosal D, Chang Y-W, Jensen GJ, Vogel JP
Journal of Bacteriology
Uncharacterized bacterial structures revealed by electron cryotomography
Dobro MJ, Oikonomou CM, Piper A, Cohen J, Guo K, Jensen T, Tadayon J, Donermeyer J, Park Y, Solis BA, Kjaer A, Jewett AI, McDowall AW, Chen S, Chang Y-W, Shi J, Subramanian P, Iancu CV, Li Z, Briegel A, Tocheva EI, Pilhofer M, Jensen GJ
Subcellular Biochemistry
The Structure, Function and Roles of the Archaeal ESCRT Apparatus
Samson RY, Dobro MJ, Jensen GJ, Bell SD
EMBO Reports
In vivo structures of an intact type VI secretion system revealed by electron cryotomography
Chang Y-W, Rettberg LA, Ortega DR, Jensen GJ
The EMBO Journal
Short FtsZ filaments can drive asymmetric cell envelope constriction at the onset of bacterial cytokinesis
Yao Q, Jewett AI, Chang Y-W, Oikonomou CM, Beeby M, Iancu CV, Briegel A, Ghosal D, Jensen GJ
Annual Review of Biochemistry
Cellular Electron Cryotomography: Toward Structural Biology In Situ
Oikonomou CM, Jensen GJ
Science
Giant viruses with an expanded complement of translation system components
Schulz F+, Yutin N, Ivanova NN, Ortega DR, Lee TK, Vierheilig J, Daims H, Horn M, Wagner M, Jensen GJ, Kyrpides NC, Koonin EV+, Woyke T+
Journal of Bacteriology
The variable internal structure of the Mycoplasma penetrans attachment organelle revealed by biochemical and microscopic analyses: implications for attachment organelle mechanism and evolution
Distelhorst SL, Jurkovic DA, Shi J, Jensen GJ, Balish MF
EMBO Reports
Structure of the Legionella Dot/Icm type IV secretion system in situ by electron cryotomography
Ghosal D, Chang Y-W, Jeong KC, Vogel JP, Jensen GJ
Annual Review of Biophysics
Progress and Potential of Electron Cryotomography as Illustrated by Its Application to Bacterial Chemoreceptor Arrays
Briegel A, Jensen G
PNAS
Polyphosphate granule biogenesis is temporally and functionally tied to cell cycle exit during starvation in Pseudomonas aeruginosa
Racki LR, Tocheva EI, Dieterle MG, Sullivan MC, Jensen GJ, Newman DK
Nature Microbiology
Architecture of the Vibrio cholerae toxin-coregulated pilus machine revealed by electron cryotomography
Chang Y-W, Kjaer A, Ortega DR, Kovacikova G, Sutherland JA, Rettberg LA, Taylor RK, Jensen GJ
Molecular Microbiology
Dynamics of the peptidoglycan biosynthetic machinery in the stalked budding bacterium Hyphomonas neptunium
Cserti E, Rosskopf S, Chang Y-W, Eisheuer S, Selter L, Shi J, Regh C, Koert U, Jensen GJ, Thanbichler M
Methods in Microbiology
Electron cryotomography
Oikonomou CM, Swulius MT, Briegel A, Beeby M, Yao Q, Chang Y-W, Jensen GJ
PNAS
Cryo-EM structure of a CD4-bound open HIV-1 envelope trimer reveals structural rearrangements of the gp120 V1V2 loop
Wang H, Cohen AA, Galimidi RP, Gristick HB, Jensen GJ, Bjorkman PJ
Molecular Microbiology
Starvation and recovery in the deep-sea methanotroph Methyloprofundus sedimenti
Tavormina PL+, Kellermann MY, Antony CP, Tocheva E, Dalleska N, Jensen AJ, Valentine DL, Hinrichs KU, Jensen G, Dubilier N, Orphan VJ
PNAS
Chemotaxis cluster 1 proteins form cytoplasmic arrays in Vibrio cholerae and are stabilized by a double signaling domain receptor DosM
Briegel A+, Ortega DR, Mann P, Kjaer A, Ringgaard S, Jensen GJ+
Nature Reviews Microbiology
Sporulation, bacterial cell envelopes, and the origin of life
Tocheva EI+, Ortega DR, Jensen GJ+
see also Reply to Comment: Tocheva et al. (2016)
Methods in Molecular Biology
Coarse-Grained Molecular Dynamics Simulations of the Bacterial Cell Wall
Nguyen LT, Gumbart JC, Jensen GJ
eLife
Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids
Li YL*, Chandrasekaran V*, Carter SD, Woodward CL, Christensen DE, Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK*, Jensen GJ*, Sundquist WI*
Nature Communications
Coarse-grained simulation reveals key features of HIV-1 capsid self-assembly
Grime JMA, Dama JF, Ganser-Pornillos BK, Woodward CL, Jensen GJ, Yeager MJ, Voth GA
ISME Journal
Phylogenomic analysis of Candidatus ‘Izimaplasma’ species: free-living representatives from a Tenericutes clade found in methane seeps
Skennerton CT+, Haroon MF, Briegel A, Shi J, Jensen GJ, Tyson GW, Orphan VJ
PNAS
Diverse high-torque bacterial flagellar motors assemble wider stator rings using a conserved protein scaffold
Beeby M, Ribardo DA, Brennan CA, Ruby EG, Jensen GJ, Hendrixson DR
Science
Architecture of the type IVa pilus machine
Chang Y-W, Rettberg LA, Treuner-Lange A, Iwasa J, Sogaard-Andersen L, Jensen GJ
Nature Reviews Microbiology
A new view into prokaryotic cell biology from electron cryotomography
Oikonomou CM, Chang Y-W, Jensen GJ
MBio
Dynamic Remodeling of the Magnetosome Membrane Is Triggered by the Initiation of Biomineralization
Cornejo E, Subramanian P, Li Z, Jensen GJ, Komeili A
PNAS
Coarse-grained simulations of bacterial cell wall growth reveal that local coordination alone can be sufficient to maintain rod shape
Nguyen LT, Gumbart JC, Beeby M, Jensen GJ
Journal of Structural Biology
The Caltech Tomography Database and Automatic Processing Pipeline
Ding HJ, Oikonomou, CM, Jensen GJ
Cellular and Molecular Life Sciences
Direct visualization of vaults within intact cells by electron cryo-tomography
Woodward CL, Mendonça LM, Jensen GJ
Environmental Microbiology Reports
Structural conservation of chemotaxis machinery across Archaea and Bacteria
Briegel A, Ortega DR, Huang AN, Oikonomou CM, Gunsalus RP, Jensen GJ
Journal of Virology
Electron cryotomography studies of maturing HIV-1 particles reveal the assembly pathway of the viral core
Woodward CL, Cheng SN, Jensen GJ
Journal of Biomolecular Screening
Streptomyces: a screening tool for bacterial cell division inhibitors
Jani C, Tocheva EI, McAuley S, Craney A, Jensen GJ, Nodwell J
Microbiology Open
Ultrastructure and complex polar architecture of the human pathogen Campylobacter jejuni
Müller A, Beeby M, McDowall AW, Chow J, Jensen GJ, Clemons WM Jr.
Nature Methods
Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography
Chang YW, Chen S, Tocheva EI, Treuner-Lange A, Löbach S, Søgaard-Andersen L, Jensen GJ
eLife
Structure of bacterial cytoplasmic chemoreceptor arrays and implications for chemotactic signaling
Briegel A, Ladinsky MS, Oikonomou C, Jones CW, Harris MJ, Fowler DJ, Chang YW, Thompson LK, Armitage JP, Jensen GJ
PLOS Computational Biology
Escherichia coli peptidoglycan structure and mechanics as predicted by atomic-scale simulations
Gumbart JC+, Beeby M, Jensen GJ, Roux B+
Biochemistry
New insights into bacterial chemoreceptor array structure and assembly from electron cryotomography
Briegel A, Wong ML, Hodges HL, Oikonomou CM, Piasta KN, Harris MJ, Fowler DJ, Thompson LK, Falke JJ, Kiessling LL, Jensen GJ
Journal of Bacteriology
Structure and expression of propanediol utilization microcompartments in Acetonema longum
Tocheva EI, Matson EG, Cheng SN, Chen WG, Leadbetter JR, Jensen GJ
Science
Marine tubeworm metamorphosis induced by arrays of bacterial phage tail-like structures
Shikuma NJ*, Pilhofer M*, Weiss GL, Hadfield MG+, Jensen GJ+, Newman DK+
Nature Communications
Discovery of chlamydial peptidoglycan reveals bacteria with murein sacculi but without FtsZ
Pilhofer M*, Aistleitner K*, Biboy J, Gray J, Kuru E, Hall E, Brun YV, VanNieuwenhze MS, Vollmer W, Horn M+, Jensen GJ+
Environmental Microbiology
Architecture and host interface of environmental chlamydiae revealed by electron cryotomography
Pilhofer M*, Aistleitner K*, Ladinsky MS, König L, Horn M+, Jensen GJ+
Chromosoma
Chromatin in a marine picoeukaryote is a disordered assemblage of nucleosomes
Gan L+, Ladinsky MS, Jensen GJ
Journal of Bacteriology
Polyphosphate storage during sporulation in the gram-negative bacterium Acetonema longum
Tocheva EI, Dekas AE, McGlynn SE, Morris D, Orphan VJ, Jensen GJ
Molecular Microbiology
The mobility of two kinase domains in the Escherichia coli chemoreceptor array varies with signalling state
Briegel A, Ames P, Gumbart JC, Oikonomou CM, Parkinson JS, Jensen GJ
Molecular Biology of the Cell
Electron cryotomography of ESCRT assemblies and dividing Sulfolobus cells suggests that spiraling filaments are involved in membrane scission
Dobro MJ, Samson RY, Yu Z, McCullough J, Ding HJ, Chong PL, Bell SD, Jensen GJ
Journal of Structural Biology
The challenge of determining handedness in electron tomography and the use of DNA origami gold nanoparticle helices as molecular standards
Briegel A*, Pilhofer M*, Mastronarde DN, Jensen GJ
Molecular Microbiology
Architecture and assembly of the Gram-positive cell wall
Beeby M, Gumbart JC, Roux B, Jensen GJ
Molecular Microbiology
Peptidoglycan transformations during Bacillus subtilis sporulation
Tocheva EI, López-Garrido J, Hughes HV, Fredlund J, Kuru E, Vannieuwenhze MS, Brun YV, Pogliano K, Jensen GJ
Nature Structural and Molecular Biology
Architecture of the major component of the type III secretion system export apparatus
Abrusci P, Vergara-Irigaray M, Johnson S, Beeby MD, Hendrixson DR, Roversi P, Friede ME, Deane JE, Jensen GJ, Tang CM, Lea SM
Journal of Molecular Biology
Unclosed HIV-1 capsids suggest a curled sheet model of assembly
Yu Z*, Dobro MJ*, Woodward CL, Levandovsky A, Danielson CM, Sandrin V, Shi J, Aiken C, Zandi R, Hope TJ, Jensen GJ
Nature Structural and Molecular Biology
Data management challenges in three-dimensional EM
Patwardhan A, Carazo JM, Carragher B, Henderson R, Heymann JB, Hill E, Jensen GJ, Lagerstedt I, Lawson CL, Ludtke SJ, Mostronarde D, Moore WJ, Roseman A, Rosenthal P, Sorzano CO, Sanz-Garcia E, Scheres SH, Subramaniam S, Westbrook J, Winn M, Swedlow JR+, Kleywegt GJ+
Cell
General protein diffusion barriers create compartments within bacterial cells
Schlimpert S*, Klein EA*, Briegel A, Hughes V, Kahnt J, Bolte K, Maier UG, Brun YV, Jensen GJ, Gitai Z, Thanbichler M
Current Opinion in Cell Biology
The bacterial cytoskeleton: more than twisted filaments
Pilhofer M, Jensen GJ
Genes and Development
A multidomain hub anchors the chromosome segregation and chemotactic machinery to the bacterial pole
Yamaichi Y*, Bruckner R*, Ringgaard S, Möll A, Cameron DE, Briegel A, Jensen GJ, Davis BM, Waldor MK
Journal of Bacteriology
The helical MreB cytoskeleton in Escherichia coli MC1000/pLE7 is an artifact of the N-Terminal yellow fluorescent protein tag
Swulius MT, Jensen GJ
Nature
Type VI secretion requires a dynamic contractile phage tail-like structure
Basler M*, Pilhofer M*, Henderson GP, Jensen GJ+, Mekalanos JJ+
PNAS
Bacterial chemoreceptor arrays are hexagonally packed trimers of receptor dimers networked by rings of kinase and coupling proteins
Briegel A*, Li X*, Bilwes AM, Hughes KT, Jensen GJ+, Crane BR+
Journal of Bacteriology
Growth and localization of polyhydroxybutyrate granules in Ralstonia eutropha
Beeby M, Cho M, Stubbe J, Jensen GJ
PLoS Biology
Microtubules in bacteria: Ancient tubulins build a five-protofilament homolog of the eukaryotic cytoskeleton
Pilhofer M+, Ladinsky MS, McDowall AW, Petroni G, Jensen GJ+
Molecular Microbiology
Activated chemoreceptor arrays remain intact and hexagonally packed
Briegel A, Beeby M, Thanbichler M, Jensen GJ
Cell
Peptidoglycan remodeling and conversion of an inner membrane into an outer membrane during sporulation
Tocheva EI, Matson EG, Morris DM, Moussavi F, Leadbetter JR, Jensen GJ
PLoS One
Nanopods: a new bacterial structure and mechanism for deployment of outer membrane vesicles
Shetty A, Chen S, Tocheva EI, Jensen GJ, Hickey WJ
The EMBO Journal
Structural diversity of bacterial flagellar motors
Chen S*, Beeby M*, Murphy GE, Leadbetter JR, Hendrixson DR, Briegel A, Li Z, Shi J, Tocheva EI, Müller A, Dobro MJ, Jensen GJ
PNAS
Alternative mechanism for bacteriophage adsorption to the motile bacterium Caulobacter crescentus
Guerrero-Ferreira RC, Viollier PH, Ely B, Poindexter JS, Georgieva M, Jensen GJ, Wright ER
Biochemical and Biophysical Research Communications
Long helical filaments are not seen encircling cells in electron cryotomograms of rod-shaped bacteria
Swulius MT, Chen S, Jane Ding H, Li Z, Briegel A, Pilhofer M, Tocheva EI, Lybarger SR, Johnson TL, Sandkvist M, Jensen GJ
Structure
In vivo assembly of an archaeal virus studied with whole-cell electron cryotomography
Fu CY, Wang K, Gan L, Lanman J, Khayat R, Young MJ, Jensen GJ, Doerschuk PC, Johnson JE
Methods in Enzymology
Correcting for the Ewald sphere in high-resolution single-particle reconstructions
Leong PA, Yu X, Zhou ZH, Jensen GJ
Methods in Enzymology
A description of the instruments, samples, protocols, and analyses that belong to the growing field of cryo-EM
Jensen GJ
Methods in Enzymology
Correlated light and electron cryo-microscopy
Briegel A, Chen S, Koster AJ, Plitzko JM, Schwartz CL, Jensen GJ
Methods in Enzymology
Plunge freezing for electron cryomicroscopy
Dobro MJ, Melanson LA, Jensen GJ, McDowall AW
Methods in Cell Biology
Bacterial TEM: new insights from cryo-microscopy
Pilhofer M, Ladinsky MS, McDowall AW, Jensen GJ
Nature Cell Biology
The metabolic enzyme CTP synthase forms cytoskeletal filaments
Ingerson-Mahar M, Briegel A, Werner JN, Jensen GJ, Gitai Z
Molecular Microbiology
DipM, a new factor required for peptidoglycan remodelling during cell division in Caulobacter crescentus
Möll A, Schlimpert S, Briegel A, Jensen GJ, Thanbichler M
JoVE
Electron cryotomography of bacterial cells
Chen S, McDowall A, Dobro MJ, Briegel A, Ladinsky M, Shi J, Tocheva EI, Beeby M, Pilhofer M, Ding HJ, Li Z, Gan L, Morris DM, Jensen GJ
Journal of Bacteriology
Mutations in the Lipopolysaccharide biosynthesis pathway interfere with crescentin-mediated cell curvature in Caulobacter crescentus
Cabeen MT*, Murolo MA*, Briegel A, Bui NK, Vollmer W, Ausmees N, Jensen GJ, Jacobs-Wagner C
The EMBO Journal
Bactofilins, a ubiquitous class of cytoskeletal proteins mediating polar localization of a cell wall synthase in Caulobacter crescentus
Kühn J, Briegel A, Mörschel E, Kahnt J, Leser K, Wick S, Jensen GJ, Thanbichler M
Journal of Molecular Biology
Organization, structure, and assembly of alpha-carboxysomes determined by electron cryotomography of intact cells
Iancu CV*, Morris DM*, Dou Z, Heinhorst S, Cannon GC, Jensen GJ
PNAS
Universal architecture of bacterial chemoreceptor arrays
Briegel A, Ortega DR, Tocheva EI, Wuichet K, Li Z, Chen S, Müller A, Iancu CV, Murphy GE, Dobro MJ, Zhulin IB, Jensen GJ
Microscopy and Microanalysis
Nanogold as a specific marker for electron cryotomography
He Y, Jensen GJ, Bjorkman PJ
Current Opinion in Microbiology
Electron cryotomography: a new view into microbial ultrastructure
Li Z, Jensen GJ
Journal of Structural Biology
Fully automated, sequential tilt-series acquisition with Leginon
Suloway C, Shi J, Cheng A, Pulokas J, Carragher B, Potter CS, Zheng SQ, Agard DA, Jensen GJ
Structure
Cryo-electron tomography of homophilic adhesion mediated by the neural cell adhesion molecule L1
He Y, Jensen GJ, Bjorkman PJ
Science
Cell biology. Protein filaments caught in the act
Jensen GJ
Comment on
Electron cryomicroscopy of E. coli reveals filament bundles involved in plasmid DNA segregation
Nature
FcRn-mediated antibody transport across epithelial cells revealed by electron tomography
He W, Ladinsky MS, Huey-Tubman KE, Jensen GJ, McIntosh JR, Björkman PJ
Cell
A self-associating protein critical for chromosome attachment, division, and polar organization in caulobacter
Ebersbach G, Briegel A, Jensen GJ, Jacobs-Wagner C
Microscopy and Microanalysis
An improved cryogen for plunge freezing
Tivol WF+, Briegel A, Jensen GJ
Journal of Structural Biology
Radiation dose reduction and image enhancement in biological imaging through equally-sloped tomography
Lee E*, Fahimian BP*, Iancu CV, Suloway C, Murphy GE, Wright ER, Castaño-Díez D, Jensen GJ, Miao J
Molecular Microbiology
Location and architecture of the Caulobacter crescentus chemoreceptor array
Briegel A, Ding HJ, Li Z, Werner J, Gitai Z, Dias DP, Jensen RB, Jensen GJ
Annual Review of Biochemistry
Toward a biomechanical understanding of whole bacterial cells
Morris DM, Jensen GJ
Journal of Molecular Biology
Cryo-EM structure of dodecameric Vps4p and its 2:1 complex with Vta1p
Yu Z, Gonciarz MD, Sundquist WI, Hill CP, Jensen GJ
Molecular Microbiology
Novel ultrastructures of Treponema primitia and their implications for motility
Murphy GE, Matson EG, Leadbetter JR, Berg HC, Jensen GJ
International Journal of Systematic and Evolutionary Microbiology
Tetrasphaera remsis sp. nov., isolated from the Regenerative Enclosed Life Support Module Simulator (REMS) air system
Osman S, Moissl C, Hosoya N, Briegel A, Mayliraj S, Satomi M, Venkateswaran K
The EMBO Journal
The structure of FtsZ filaments in vivo suggests a force-generating role in cell division
Li Z, Trimble MJ, Brun YV, Jensen GJ
PLoS One
3-D ultrastructure of O. tauri: electron cryotomography of an entire eukaryotic cell
Henderson GP, Gan L, Jensen GJ
Journal of Molecular Biology
The structure of isolated Synechococcus strain WH8102 carboxysomes as revealed by electron cryotomography
Iancu CV, Ding HJ, Morris DM, Dias DP, Gonzales AD, Martino A, Jensen GJ
Current Opinion in Structural Biology
How electron cryotomography is opening a new window onto prokaryotic ultrastructure
Jensen GJ, Briegel A
The EMBO Journal
Electron cryotomography of immature HIV-1 virions reveals the structure of the CA and SP1 Gag shells
Wright ER*, Schooler JB*, Ding HJ, Kieffer C, Fillmore C, Sundquist WI, Jensen GJ
Int’l Journal of Systematic & Evolutionary Microbiology
Haloquadratum walsbyi gen. nov., sp. nov., the square haloarchaeon of Walsby, isolated from saltern crystallizers in Australia and Spain
Burns DG, Janssen PH, Itoh T, Kamekura M, Li Z, Jensen G, Rodriguez-Valera F, Bolhuis H, Dyall-Smith ML
Nature
In situ structure of the complete Treponema primitia flagellar motor
Murphy GE, Leadbetter JR, Jensen GJ
Molecular Microbiology
Multiple large filament bundles observed in Caulobacter crescentus by electron cryotomography
Briegel A, Dias DP, Li Z, Jensen RB, Frangakis AS, Jensen GJ
Nature Protocols
Electron cryotomography sample preparation using the Vitrobot
Iancu CV, Tivol WF, Schooler JB, Dias DP, Henderson GP, Murphy GE, Wright ER, Li Z, Yu Z, Briegel A, Gan L, He Y, Jensen GJ
Molecular Microbiology
Three-dimensional structure of Mycoplasma pneumoniae’s attachment organelle and a model for its role in gliding motility
Henderson GP, Jensen GJ
Journal of the American Chemical Society
Rigid, specific, and discrete gold nanoparticle/antibody conjugates
Ackerson CJ, Jadzinsky PD, Jensen GJ, Kornberg RD
Journal of Structural Biology
Observations on the behavior of vitreous ice at approximately 82 and approximately 12 K
Wright ER*, Iancu CV*, Tivol WF, Jensen GJ
Journal of Structural Biology
A comparison of liquid nitrogen and liquid helium as cryogens for electron cryotomography
Iancu CV, Wright ER, Heymann JB, Jensen GJ
Science
Magnetosomes are cell membrane invaginations organized by the actin-like protein MamK
Komeili A*+, Li Z*, Newman DK, Jensen GJ
Structure
Electron cryotomography of the E. coli pyruvate and 2-oxoglutarate dehydrogenase complexes
Murphy GE, Jensen GJ
Journal of Structural Biology
A “flip-flop” rotation stage for routine dual-axis electron cryotomography
Iancu CV*, Wright ER*, Benjamin J, Tivol WF, Dias DP, Murphy GE, Morrison RC, Heymann JB, Jensen GJ
Structure
Peach: a simple Perl-based system for distributed computation and its application to cryo-EM data processing
Leong PA*, Heymann JB*, Jensen GJ
Journal of Molecular Biology
Three-dimensional structure of HIV-1 virus-like particles by electron cryotomography
Benjamin J, Ganser-Pornillos BK, Tivol WF, Sundquist WI, Jensen GJ