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Structure of Anabaena flos-aquae gas vesicles revealed by cryo-ET

Dutka P, Metskas LA, Hurt RC, Salahshoor H, Wang T-Y, Malounda D, Lu G, Chou T-F, Shapiro MG+, Jensen GJ+


Dynamic structural adaptations enable the endobiotic predation of Bdellovibrio bacteriovorus

Kaplan M*, Chang Y-W*, Oikonomou CM, Nicolas WJ, Jewett AI, Kreida S, Dutka P, Rettberg LA, Maggi S, Jensen GJ


Journal of Structural Biology

Montage electron tomography of vitrified specimens

Peck A, Carter SD, Mai H, Chen S, Burt A, Jensen GJ

EMBO Journal

Novel transient cytoplasmic rings stabilize assembling bacterial flagellar motors

Kaplan M, Oikonomou CM, Wood CR, Chreifi G, Subramanian P, Ortega DR, Chang Y-W, Beeby M, Shaffer CL, Jensen GJ


Rubisco forms a lattice inside alpha-carboxysomes

Metskas LA, Ortega D, Oltrogge LM, Blikstad C, Laughlin T, Savage DF, Jensen GJ

Communications Biology

UVC inactivation of pathogenic samples suitable for cryo-EM analysis

Depelteau JS, Renault L, Althof N, Cassidy CK, Mendonça LM, Jensen GJ, Resch GP, Briegel A




Nanoscale details of mitochondrial fission revealed by cryo-electron tomography

Mageswaran SK*, Grotjahn DA*+, Zeng X, Barad BA, Medina M, Hoang MH, Dobro MJ, Chang Y-W, Xu M, Yang WY, Jensen GJ+

Research Square

Archaic chaperone-usher pilus self-secretes into a superelastic zigzag spring architecture

Zavialov A, Pakharukova N, Malmi H, Tuittila M, Paavilainen S, Dahlberg T, Andersson M, Myint SL, Uhlin BE, Ghosal D, Chang Y-W, Jensen GJ, Knight S, Lamminmäki U


A bacterial membrane sculpting protein with BAR domain-like activity

Phillips DA*, Zacharoff LA*, Hampton CM, Chong GW, Malanoski AP, Metskas LA, Xu S, Bird LJ, Eddie BJ, Miklos AE, Jensen GJ, Drummy LF, El-Naggar MY, Glaven SM


The stress-sensing domain of activated IRE1α forms helical filaments in narrow ER membrane tubes

Tran N-H*, Carter SD*, De Maziére A, Ashkenazi A, Klumperman J, Walter P+, Jensen GJ+

Journal of Microbiology & Biology Education

The Atlas of Bacterial & Archaeal Cell Structure: an interactive open-access microbiology textbook

Oikonomou CM, Jensen GJ


A novel widespread bacterial structure related to the flagellar type III secretion system

Kaplan M, Oikonomou CM, Wood CR, Chreifi G, Ghosal D, Dobro MJ, Yao Q, McDowall A, Briegel A, Beeby M, Chang Y-W, Shaffer CL, Jensen GJ


In situ imaging of bacterial membrane projections and associated protein complexes using electron cryo-tomography

Kaplan M, Chreifi G, Metskas LA, Liedtke J, Wood CR, Oikonomou CM, Nicolas WJ, Subramanian P, Zacharoff LA, Wang Y, Chang Y-W, Beeby M, Dobro M, Zhu Y, McBride MJ, Briegel A, Shaffer C, Jensen GJ


Loss of the bacterial flagellar motor switch complex upon cell lysis

Kaplan M, Tocheva EI, Briegel A, Dobro MJ, Chang Y-W, Subramanian P, McDowall AW, Beeby M, Jensen GJ


Generation of ordered protein assemblies using rigid three-body fusion

Vulovic I, Yao Q*, Park Y-J*, Courbet A, Norris A, Busch F, Sahasrabuddhe A, Merten H, Sahtoe DD, Ueda G, Fallas JA, Weaver SJ, Hsia Y, Langan RA, Plückthun A, Wysocki VH, Veesler D, Jensen GJ, Baker D

Acta Crystallographica Section D

Challenges in solving structures from radiation-damaged tomograms of protein nanocrystals assessed by simulation

Peck A, Yao Q, Brewster AS, Zwart PH, Heumann JM, Sauter NK, Jensen GJ

Journal of Molecular Biology

Programmed flagellar ejection in Caulobacter crescentus leaves PL-subcomplexes

Kaplan M, Wang Y, Chreifi G, Zhang L, Chang Y-W, Jensen GJ

Science Advances

A cryo-electron tomography workflow reveals protrusion-mediated shedding on injured plasma membrane

Mageswaran SK, Yang WY+, Chakrabarty Y, Oikonomou CM, Jensen GJ+

Protein Science

Measuring gas vesicle dimensions by electron microscopy

Dutka P, Malounda D, Metskas LA, Chen S, Hurt RC, Lu GJ, Jensen GJ+, Shapiro MK+

Journal of Molecular Biology

Moltemplate: a tool for coarse-grained modeling of complex biological matter and soft condensed matter physics

Jewett AI+, Stelter D, Lambert J, Saladi SM, Roscioni OM, Ricci M, Autin L, Maritan M, Bashusqeh SM, Keyes T, Dame RT, Shea J-E, Jensen GJ, Goodsell DS+



Methods in Molecular Biology: CryoEM

In situ imaging and structure determination of biomolecular complexes using electron cryo-tomography

Kaplan M, Nicolas WJ, Zhao W, Carter SD, Metskas LA, Chreifi G, Ghosal D, Jensen GJ

Science Advances

Visualizing insulin vesicle neighborhoods in β cells by cryo-electron tomography

Zhang X, Carter SD, Singla J, White KL, Butler PC, Stevens RC+, Jensen GJ+

Journal of Bacteriology

Simulations of proposed mechanisms of FtsZ-driven cell constriction

Nguyen LT, Oikonomou CM, Jensen GJ

Journal of Bacteriology

Structure of the bacterial cellulose ribbon and its assembly-guiding cytoskeleton by electron cryotomography

Nicolas WJ, Ghosal D, Tocheva EI, Meyerowitz EM, Jensen GJ

Nature Communications

CryoEM structure of the Type IVa pilus secretin required for natural competence in Vibrio cholerae

Weaver SJ, Ortega DR, Sazinsky MH, Dalia TN, Dalia AB, Jensen GJ

Nature Communications

PilY1 and minor pilins form a complex priming the type IVa pilus in Myxococcus xanthus

Treuner-Lange A, Chang Y-W, Glatter T, Herfurth M, Lindow S, Chreifi G, Jensen GJ, Søgaard-Andersen L

Photodiagnosis and Photodynamic Therapy

Effects of antimicrobial photodynamic therapy on antibiotic-resistant Escherichia coli

Garcez AS*+, Kaplan M*, Jensen GJ, Scheidt FR, Oliveira EM, Suzuki SS


In situ structure and organisation of the type IX secretion system

Gorasia DG*, Chreifi G*, Seers CA, Butler CA, Heath JE, Glew MD, McBride MJ, Subramanian P, Kjaer A, Jensen GJ+, Veith PD+, Reynolds EC+

Nature Communications

Repurposing a chemosensory macromolecular machine

Ortega DR, Yang W, Subramanian P, Mann P, Kjær A, Chen S, Watts KJ, Pirbadian A, Collins DA, Kooger R, Kalyuzhnaya MG, Ringgaard S, Briegel A+, Jensen GJ+


Bacterial flagellar motor PL-ring disassembly subcomplexes are widespread and ancient

Kaplan M, Sweredoski MJ, Rodrigues JPGLM, Tocheva EI, Chang Y-W, Ortega DR, Beeby M, Jensen GJ

Science Advances

Ribosome-associated vesicles: A dynamic subcompartment of the endoplasmic reticulum in secretory cells

Carter SD*, Hampton CM*, Langlois R*, Melero R, Farino ZJ, Calderon MJ, Li W, Wallace CT, Tran NH, Grassucci RA, Siegmund SE, Pemberton J, Morgenstern TJ, Eisenman L, Aguilar JI, Greenberg NL, Levy ES, Yi E, Mitchell WG, Rice WJ, Wigge C, Pilli J, George EW, Aslanoglou D, Courel M, Freyberg RJ, Javitch JA, Wills ZP, Area-Gomez E, Shiva S, Bartolini F, Volchuk A, Murray SA, Aridor M, Fish KN, Walter P, Balla T, Fass D, Wolf SG, Watkins SC, Carazo JM, Jensen GJ+, Frank J+, Freyberg Z+



Nature Microbiology

In vivo structure of the Legionella type II secretion system by electron cryotomography

Ghosal D, Kim KW, Zheng H, Kaplan M, Truchan HK, Lopez AE, McIntire IE, Vogel JP, Cianciotto NP, Jensen GJ

Gateways 2019 (Conference)

DDX-Interface: A factory of interoperable scientific gateways

James A, Chacon R, Buddenhagen J, Ortega DR, Rees-Lee P, Jensen G, James DR


Distinct chemotaxis protein paralogs assemble into chemoreceptor signaling arrays to coordinate signaling output

O’Neal L*, Gullett JM*, Aksenova A, Hubler A, Briegel A, Ortega D, Kjaer A, Jensen G, Alexandre G


Qualitative analyses of polishing and precoating FIB milled crystals for MicroED

Martynowycz MW, Zhao W, Hattne J, Jensen GJ, Gonen T

EMBO Journal

In situ imaging of the bacterial flagellar motor disassembly and assembly processes

Kaplan M, Subramanian P, Ghosal D, Oikonomou CM, Pirbadian S, Starwalt-Lee R, Mageswaran SK, Ortega DR, Gralnick JA, El-Naggar MY, Jensen GJ


Fusion of DARPin to aldolase enables visualization of small protein by cryoEM

Yao Q*, Weaver SJ*, Mock J-Y, Jensen GJ

Nature Microbiology

Molecular architecture, polar targeting and biogenesis of the Legionella Dot/Icm T4SS

Ghosal D*, Jeong KC*, Chang Y-W, Gyore J, Teng L, Gardner A, Vogel JP+, Jensen GJ+


ETDB-Caltech: a blockchain-based distributed public database for electron tomography

Ortega DR, Oikonomou CM, Ding HJ, Rees-Lee P, Alexandria, Jensen GJ

Microbiology Spectrum

Electron cryotomography of bacterial secretion systems

Oikonomou CM, Jensen GJ

Nature Communications

Simulations suggest a constrictive force is required for Gram-negative bacterial cell division

Nguyen LT, Oikonomou CM, Ding HJ, Kaplan M, Yao Q, Chang Y-W, Beeby M, Jensen GJ


De novo structural pattern mining in cellular electron cryotomograms

Xu M, Singla J, Tocheva EI, Chang Y-W, Stevens RC, Jensen GJ, Alber F


FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles

Azubel M, Carter SD, Weiszmann J, Zhang J, Jensen GJ, Li Y, Kornberg RD


Collection of continuous rotation MicroED data from ion beam-milled crystals of any size

Martynowycz MW, Zhao W, Hattne J, Jensen GJ, Gonen T


The presence and absence of periplasmic rings in bacterial flagellar motors correlates with stator type

Kaplan M, Ghosal D, Subramanian P, Oikonomou CM, Kjaer A, Pirbadian S, Ortega DR, Briegel A, El-Naggar MY, Jensen GJ

Journal of Structural Biology

Rapid tilt-series acquisition for electron cryotomography

Chreifi G*, Chen S*, Metskas LA, Kaplan M, Jensen GJ




Programmed secretion arrest and receptor-triggered toxin export during antibacterial contact-dependent growth inhibition

Ruhe ZC*, Subramanian P*, Song K*, Nguyen JY, Stevens TA, Low DA, Jensen GJ+, Hayes CS+


Three-dimensional analysis of mitochondrial crista ultrastructure in a patient with Leigh Syndrome by in situ cryoelectron tomography

Siegmund SE, Grassucci R, Carter SD, Barca E, Farino ZJ, Juanola-Falgarona M, Zhang P, Tanji K, Hirano M, Schon EA, Frank J, Freyberg Z

Journal of Physical Chemistry B

Selective permeability of carboxysome shell pores to anionic molecules

Mahinthichaichan P*, Morris DM*, Wang Y, Jensen GJ+, Tajkhorshid E+

ISME Journal

Nutrient transport suggests an evolutionary basis for charged archaeal surface layer proteins

Li P-N, Herrmann J, Tolar BB, Poitevin F, Ramdasi R, Bargar JR, Stahl DA, Jensen GJ, Francis CA+, Wakatsuki S+, van den Bedem H+

Molecular Biology of the Cell

Coarse-grained simulations of actomyosin rings point to a nodeless model involving both unipolar and bipolar myosins

Nguyen LT, Swulius MT, Aich S, Mishra M, Jensen GJ

Cell Reports

In vivo structures of the Helicobacter pylori cag type IV secretion system

Chang Y-W*, Shaffer CL*, Rettberg LA, Ghosal D, Jensen GJ


Ultrastructure of Shewanella oneidensis MR-1 nanowires revealed by electron cryotomography

Subramanian P*, Pirbadian S*, El-Naggar MY+, Jensen GJ+

Molecular Microbiology

Electron cryotomography of Mycoplasma pneumoniae mutants correlates terminal organelle architectural features and function

Krause DC+, Chen S, Shi J, Jensen A, Sheppard ES, Jensen GJ


Structure of the fission yeast actomyosin ring during constriction

Swulius MT*, Nguyen LT*, Ladinsky MS, Ortega DR, Aich S, Mishra M, Jensen GJ




Assigning chemoreceptors to chemosensory pathways in Pseudomonas aeruginosa

Ortega DR*, Fleetwood AD*, Krell T, Harwood CS, Jensen GJ, Zhulin IB

Journal of Structural Biology

Distinguishing signal from autofluorescence in cryogenic correlated light and electron microscopy of mammalian cells

Carter SD*, Mageswaran SK*, Farino ZJ, Mamede JI, Oikonomou CM, Hope TJ, Freyberg Z+, Jensen GJ+

International Journal of Systematic & Evolutionary Microbiology

Nitrosopumilus maritimus gen. nov., sp. nov., Nitrosopumilus cobalaminigenes sp. nov., Nitrosopumilus oxyclinae sp. nov., and Nitrosopumilus ureiphilus sp. nov., four marine ammonia-oxidizing archaea of the phylum Thaumarchaeota

Qin W, Heal KR, Ramdasi R, Kobelt JN, Martens-Habbena W, Bertagnolli AD, Amin SA, Walker CB, Urakawa H, Könneke M, Devol AH, Moffett JW, Armbrust EV, Jensen GJ, Ingalls AE, Stahl DA

Molecular Microbiology

LytM factors affect the recruitment of autolysins to the cell division site in Caulobacter crescentus

Zielińska A*, Billini M*, Möll A, Kremer K, Briegel A, Martinez AI, Jensen GJ, Thanbichler M

Journal of Structural Biology

Best practices for managing large cryoEM facilities

Alewijnse B, Ashton AW, Chambers MG, Chen S, Cheng A, Ebrahim M, Eng E, Hagen WJH, Koster AJ, Lopez CS, Lukoyanova N, Ortega J, Renault L, Reyntjens S, Rice WJ, Scapin G, Schrijver R, Siebert A, Stagg SM, Grum-Tokars V, Wright ER, Wu S, Yu Z, Zhou ZH, Carragher B, Potter CS

Angewandte Chemie International Edition

Photon-induced near-field electron microscopy (PINEM) of eukaryotic cells

Kaplan M, Yoo BK, Tang J+, Karam T, Liao B, Majumdar B, Baltimore D, Jensen G+, Zewail A

EMBO Reports

Morphology of the archaellar motor and associated cytoplasmic cone in Thermococcus kodakaraensis

Briegel A, Oikonomou CM, Chang Y-W, Kjaer A, Huang AN, Kim KW, Ghosal D, Nguyen HH, Kenny D, Ogorzalek Loo RR, Gunsalus RP, Jensen GJ

Journal of Bacteriology

Uncharacterized bacterial structures revealed by electron cryotomography

Dobro MJ, Oikonomou CM, Piper A, Cohen J, Guo K, Jensen T, Tadayon J, Donermeyer J, Park Y, Solis BA, Kjaer A, Jewett AI, McDowall AW, Chen S, Chang Y-W, Shi J, Subramanian P, Iancu CV, Li Z, Briegel A, Tocheva EI, Pilhofer M, Jensen GJ

Subcellular Biochemistry

The Structure, Function and Roles of the Archaeal ESCRT Apparatus

Samson RY, Dobro MJ, Jensen GJ, Bell SD

The EMBO Journal

Short FtsZ filaments can drive asymmetric cell envelope constriction at the onset of bacterial cytokinesis

Yao Q, Jewett AI, Chang Y-W, Oikonomou CM, Beeby M, Iancu CV, Briegel A, Ghosal D, Jensen GJ

Annual Review of Biochemistry

Cellular Electron Cryotomography: Toward Structural Biology In Situ

Oikonomou CM, Jensen GJ


Giant viruses with an expanded complement of translation system components

Schulz F+, Yutin N, Ivanova NN, Ortega DR, Lee TK, Vierheilig J, Daims H, Horn M, Wagner M, Jensen GJ, Kyrpides NC, Koonin EV+, Woyke T+

EMBO Reports

Structure of the Legionella Dot/Icm type IV secretion system in situ by electron cryotomography

Ghosal D, Chang Y-W, Jeong KC, Vogel JP, Jensen GJ

Nature Microbiology

Architecture of the Vibrio cholerae toxin-coregulated pilus machine revealed by electron cryotomography

Chang Y-W, Kjaer A, Ortega DR, Kovacikova G, Sutherland JA, Rettberg LA, Taylor RK, Jensen GJ



Molecular Microbiology

Dynamics of the peptidoglycan biosynthetic machinery in the stalked budding bacterium Hyphomonas neptunium

Cserti E, Rosskopf S, Chang Y-W, Eisheuer S, Selter L, Shi J, Regh C, Koert U, Jensen GJ, Thanbichler M

Methods in Microbiology

Electron cryotomography

Oikonomou CM, Swulius MT, Briegel A, Beeby M, Yao Q, Chang Y-W, Jensen GJ

(proof available here)

Molecular Microbiology

Starvation and recovery in the deep-sea methanotroph Methyloprofundus sedimenti

Tavormina PL+, Kellermann MY, Antony CP, Tocheva E, Dalleska N, Jensen AJ, Valentine DL, Hinrichs KU, Jensen G, Dubilier N, Orphan VJ

Methods in Molecular Biology

Coarse-Grained Molecular Dynamics Simulations of the Bacterial Cell Wall

Nguyen LT, Gumbart JC, Jensen GJ


Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids

Li YL*, Chandrasekaran V*, Carter SD, Woodward CL, Christensen DE, Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK*, Jensen GJ*, Sundquist WI*

Nature Communications

Coarse-grained simulation reveals key features of HIV-1 capsid self-assembly

Grime JMA, Dama JF, Ganser-Pornillos BK, Woodward CL, Jensen GJ, Yeager MJ, Voth GA


Diverse high-torque bacterial flagellar motors assemble wider stator rings using a conserved protein scaffold

Beeby M, Ribardo DA, Brennan CA, Ruby EG, Jensen GJ, Hendrixson DR


Architecture of the type IVa pilus machine

Chang Y-W, Rettberg LA, Treuner-Lange A, Iwasa J, Sogaard-Andersen L, Jensen GJ

Nature Reviews Microbiology

A new view into prokaryotic cell biology from electron cryotomography

Oikonomou CM, Chang Y-W, Jensen GJ



Journal of Structural Biology

The Caltech Tomography Database and Automatic Processing Pipeline

Ding HJ, Oikonomou, CM, Jensen GJ

Cellular and Molecular Life Sciences

Direct visualization of vaults within intact cells by electron cryo-tomography

Woodward CL, Mendonça LM, Jensen GJ

Environmental Microbiology Reports

Structural conservation of chemotaxis machinery across Archaea and Bacteria

Briegel A, Ortega DR, Huang AN, Oikonomou CM, Gunsalus RP, Jensen GJ

Journal of Biomolecular Screening

Streptomyces: a screening tool for bacterial cell division inhibitors

Jani C, Tocheva EI, McAuley S, Craney A, Jensen GJ, Nodwell J



Microbiology Open

Ultrastructure and complex polar architecture of the human pathogen Campylobacter jejuni

Müller A, Beeby M, McDowall AW, Chow J, Jensen GJ, Clemons WM Jr.

Nature Methods

Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography

Chang YW, Chen S, Tocheva EI, Treuner-Lange A, Löbach S, Søgaard-Andersen L, Jensen GJ


Structure of bacterial cytoplasmic chemoreceptor arrays and implications for chemotactic signaling

Briegel A, Ladinsky MS, Oikonomou C, Jones CW, Harris MJ, Fowler DJ, Chang YW, Thompson LK, Armitage JP, Jensen GJ

PLOS Computational Biology

Escherichia coli peptidoglycan structure and mechanics as predicted by atomic-scale simulations

Gumbart JC+, Beeby M, Jensen GJ, Roux B+


New insights into bacterial chemoreceptor array structure and assembly from electron cryotomography

Briegel A, Wong ML, Hodges HL, Oikonomou CM, Piasta KN, Harris MJ, Fowler DJ, Thompson LK, Falke JJ, Kiessling LL, Jensen GJ

Journal of Bacteriology

Structure and expression of propanediol utilization microcompartments in Acetonema longum

Tocheva EI, Matson EG, Cheng SN, Chen WG, Leadbetter JR, Jensen GJ


Marine tubeworm metamorphosis induced by arrays of bacterial phage tail-like structures

Shikuma NJ*, Pilhofer M*, Weiss GL, Hadfield MG+, Jensen GJ+, Newman DK+



Nature Communications

Discovery of chlamydial peptidoglycan reveals bacteria with murein sacculi but without FtsZ

Pilhofer M*, Aistleitner K*, Biboy J, Gray J, Kuru E, Hall E, Brun YV, VanNieuwenhze MS, Vollmer W, Horn M+, Jensen GJ+

Environmental Microbiology

Architecture and host interface of environmental chlamydiae revealed by electron cryotomography

Pilhofer M*, Aistleitner K*, Ladinsky MS, König L, Horn M+, Jensen GJ+

Journal of Bacteriology

Polyphosphate storage during sporulation in the gram-negative bacterium Acetonema longum

Tocheva EI, Dekas AE, McGlynn SE, Morris D, Orphan VJ, Jensen GJ

Molecular Microbiology

The mobility of two kinase domains in the Escherichia coli chemoreceptor array varies with signalling state

Briegel A, Ames P, Gumbart JC, Oikonomou CM, Parkinson JS, Jensen GJ

Molecular Biology of the Cell

Electron cryotomography of ESCRT assemblies and dividing Sulfolobus cells suggests that spiraling filaments are involved in membrane scission

Dobro MJ, Samson RY, Yu Z, McCullough J, Ding HJ, Chong PL, Bell SD, Jensen GJ

Molecular Microbiology

Architecture and assembly of the Gram-positive cell wall

Beeby M, Gumbart JC, Roux B, Jensen GJ

Molecular Microbiology

Peptidoglycan transformations during Bacillus subtilis sporulation

Tocheva EI, López-Garrido J, Hughes HV, Fredlund J, Kuru E, Vannieuwenhze MS, Brun YV, Pogliano K, Jensen GJ

Nature Structural and Molecular Biology

Architecture of the major component of the type III secretion system export apparatus

Abrusci P, Vergara-Irigaray M, Johnson S, Beeby MD, Hendrixson DR, Roversi P, Friede ME, Deane JE, Jensen GJ, Tang CM, Lea SM

Journal of Molecular Biology

Unclosed HIV-1 capsids suggest a curled sheet model of assembly

Yu Z*, Dobro MJ*, Woodward CL, Levandovsky A, Danielson CM, Sandrin V, Shi J, Aiken C, Zandi R, Hope TJ, Jensen GJ



Nature Structural and Molecular Biology

Data management challenges in three-dimensional EM

Patwardhan A, Carazo JM, Carragher B, Henderson R, Heymann JB, Hill E, Jensen GJ, Lagerstedt I, Lawson CL, Ludtke SJ, Mostronarde D, Moore WJ, Roseman A, Rosenthal P, Sorzano CO, Sanz-Garcia E, Scheres SH, Subramaniam S, Westbrook J, Winn M, Swedlow JR+, Kleywegt GJ+


General protein diffusion barriers create compartments within bacterial cells

Schlimpert S*, Klein EA*, Briegel A, Hughes V, Kahnt J, Bolte K, Maier UG, Brun YV, Jensen GJ, Gitai Z, Thanbichler M

Current Opinion in Cell Biology

The bacterial cytoskeleton: more than twisted filaments

Pilhofer M, Jensen GJ

Genes and Development

A multidomain hub anchors the chromosome segregation and chemotactic machinery to the bacterial pole

Yamaichi Y*, Bruckner R*, Ringgaard S, Möll A, Cameron DE, Briegel A, Jensen GJ, Davis BM, Waldor MK


Type VI secretion requires a dynamic contractile phage tail-like structure

Basler M*, Pilhofer M*, Henderson GP, Jensen GJ+, Mekalanos JJ+

Journal of Bacteriology

Growth and localization of polyhydroxybutyrate granules in Ralstonia eutropha

Beeby M, Cho M, Stubbe J, Jensen GJ

Quarterly Reviews of Biophysics

Electron Tomography of Cells

Gan L, Jensen GJ



PLoS Biology

Microtubules in bacteria: Ancient tubulins build a five-protofilament homolog of the eukaryotic cytoskeleton

Pilhofer M+, Ladinsky MS, McDowall AW, Petroni G, Jensen GJ+

Molecular Microbiology

Activated chemoreceptor arrays remain intact and hexagonally packed

Briegel A, Beeby M, Thanbichler M, Jensen GJ

Current Biology

Organization of the smallest eukaryotic spindle

Gan L, Ladinsky MS, Jensen GJ


Peptidoglycan remodeling and conversion of an inner membrane into an outer membrane during sporulation

Tocheva EI, Matson EG, Morris DM, Moussavi F, Leadbetter JR, Jensen GJ

PLoS One

Nanopods: a new bacterial structure and mechanism for deployment of outer membrane vesicles

Shetty A, Chen S, Tocheva EI, Jensen GJ, Hickey WJ

The EMBO Journal

Structural diversity of bacterial flagellar motors

Chen S*, Beeby M*, Murphy GE, Leadbetter JR, Hendrixson DR, Briegel A, Li Z, Shi J, Tocheva EI, Müller A, Dobro MJ, Jensen GJ


Alternative mechanism for bacteriophage adsorption to the motile bacterium Caulobacter crescentus

Guerrero-Ferreira RC, Viollier PH, Ely B, Poindexter JS, Georgieva M, Jensen GJ, Wright ER

Biochemical and Biophysical Research Communications

Long helical filaments are not seen encircling cells in electron cryotomograms of rod-shaped bacteria

Swulius MT, Chen S, Jane Ding H, Li Z, Briegel A, Pilhofer M, Tocheva EI, Lybarger SR, Johnson TL, Sandkvist M, Jensen GJ




In vivo assembly of an archaeal virus studied with whole-cell electron cryotomography

Fu CY, Wang K, Gan L, Lanman J, Khayat R, Young MJ, Jensen GJ, Doerschuk PC, Johnson JE

Methods in Enzymology

Correcting for the Ewald sphere in high-resolution single-particle reconstructions

Leong PA, Yu X, Zhou ZH, Jensen GJ

Methods in Enzymology

Cryo-EM, Part B: 3-D Reconstruction

Jensen GJ

Methods in Enzymology

Correlated light and electron cryo-microscopy

Briegel A, Chen S, Koster AJ, Plitzko JM, Schwartz CL, Jensen GJ

Methods in Enzymology

Plunge freezing for electron cryomicroscopy

Dobro MJ, Melanson LA, Jensen GJ, McDowall AW

Methods in Cell Biology

Bacterial TEM: new insights from cryo-microscopy

Pilhofer M, Ladinsky MS, McDowall AW, Jensen GJ

Nature Cell Biology

The metabolic enzyme CTP synthase forms cytoskeletal filaments

Ingerson-Mahar M, Briegel A, Werner JN, Jensen GJ, Gitai Z

Cold Spring Harbor Perspectives in Biology

Electron cryotomography

Tocheva EI, Li Z, Jensen GJ

Molecular Microbiology

DipM, a new factor required for peptidoglycan remodelling during cell division in Caulobacter crescentus

Möll A, Schlimpert S, Briegel A, Jensen GJ, Thanbichler M


Electron cryotomography of bacterial cells

Chen S, McDowall A, Dobro MJ, Briegel A, Ladinsky M, Shi J, Tocheva EI, Beeby M, Pilhofer M, Ding HJ, Li Z, Gan L, Morris DM, Jensen GJ

Journal of Bacteriology

Mutations in the Lipopolysaccharide biosynthesis pathway interfere with crescentin-mediated cell curvature in Caulobacter crescentus

Cabeen MT*, Murolo MA*, Briegel A, Bui NK, Vollmer W, Ausmees N, Jensen GJ, Jacobs-Wagner C

The EMBO Journal

Bactofilins, a ubiquitous class of cytoskeletal proteins mediating polar localization of a cell wall synthase in Caulobacter crescentus

Kühn J, Briegel A, Mörschel E, Kahnt J, Leser K, Wick S, Jensen GJ, Thanbichler M

Journal of Molecular Biology

Organization, structure, and assembly of alpha-carboxysomes determined by electron cryotomography of intact cells

Iancu CV*, Morris DM*, Dou Z, Heinhorst S, Cannon GC, Jensen GJ




Universal architecture of bacterial chemoreceptor arrays

Briegel A, Ortega DR, Tocheva EI, Wuichet K, Li Z, Chen S, Müller A, Iancu CV, Murphy GE, Dobro MJ, Zhulin IB, Jensen GJ

Microscopy and Microanalysis

Nanogold as a specific marker for electron cryotomography

He Y, Jensen GJ, Bjorkman PJ

Current Opinion in Microbiology

Electron cryotomography: a new view into microbial ultrastructure

Li Z, Jensen GJ

Journal of Structural Biology

Fully automated, sequential tilt-series acquisition with Leginon

Suloway C, Shi J, Cheng A, Pulokas J, Carragher B, Potter CS, Zheng SQ, Agard DA, Jensen GJ


Cell biology. Protein filaments caught in the act

Jensen GJ

Comment on

Electron cryomicroscopy of E. coli reveals filament bundles involved in plasmid DNA segregation




FcRn-mediated antibody transport across epithelial cells revealed by electron tomography

He W, Ladinsky MS, Huey-Tubman KE, Jensen GJ, McIntosh JR, Björkman PJ

Microscopy and Microanalysis

An improved cryogen for plunge freezing

Tivol WF+, Briegel A, Jensen GJ

Journal of Structural Biology

Radiation dose reduction and image enhancement in biological imaging through equally-sloped tomography

Lee E*, Fahimian BP*, Iancu CV, Suloway C, Murphy GE, Wright ER, Castaño-Díez D, Jensen GJ, Miao J

Molecular Microbiology

Location and architecture of the Caulobacter crescentus chemoreceptor array

Briegel A, Ding HJ, Li Z, Werner J, Gitai Z, Dias DP, Jensen RB, Jensen GJ

Annual Review of Biochemistry

Toward a biomechanical understanding of whole bacterial cells

Morris DM, Jensen GJ

Journal of Molecular Biology

Cryo-EM structure of dodecameric Vps4p and its 2:1 complex with Vta1p

Yu Z, Gonciarz MD, Sundquist WI, Hill CP, Jensen GJ

Molecular Microbiology

Novel ultrastructures of Treponema primitia and their implications for motility

Murphy GE, Matson EG, Leadbetter JR, Berg HC, Jensen GJ



International Journal of Systematic and Evolutionary Microbiology

Tetrasphaera remsis sp. nov., isolated from the Regenerative Enclosed Life Support Module Simulator (REMS) air system

Osman S, Moissl C, Hosoya N, Briegel A, Mayliraj S, Satomi M, Venkateswaran K


Electron cryotomography

Murphy GE, Jensen GJ

Journal of Molecular Biology

The structure of isolated Synechococcus strain WH8102 carboxysomes as revealed by electron cryotomography

Iancu CV, Ding HJ, Morris DM, Dias DP, Gonzales AD, Martino A, Jensen GJ

Current Opinion in Structural Biology

How electron cryotomography is opening a new window onto prokaryotic ultrastructure

Jensen GJ, Briegel A

The EMBO Journal

Electron cryotomography of immature HIV-1 virions reveals the structure of the CA and SP1 Gag shells

Wright ER*, Schooler JB*, Ding HJ, Kieffer C, Fillmore C, Sundquist WI, Jensen GJ

Int’l Journal of Systematic & Evolutionary Microbiology

Haloquadratum walsbyi gen. nov., sp. nov., the square haloarchaeon of Walsby, isolated from saltern crystallizers in Australia and Spain

Burns DG, Janssen PH, Itoh T, Kamekura M, Li Z, Jensen G, Rodriguez-Valera F, Bolhuis H, Dyall-Smith ML



Molecular Microbiology

Multiple large filament bundles observed in Caulobacter crescentus by electron cryotomography

Briegel A, Dias DP, Li Z, Jensen RB, Frangakis AS, Jensen GJ

Nature Protocols

Electron cryotomography sample preparation using the Vitrobot

Iancu CV, Tivol WF, Schooler JB, Dias DP, Henderson GP, Murphy GE, Wright ER, Li Z, Yu Z, Briegel A, Gan L, He Y, Jensen GJ

Journal of the American Chemical Society

Rigid, specific, and discrete gold nanoparticle/antibody conjugates

Ackerson CJ, Jadzinsky PD, Jensen GJ, Kornberg RD

Journal of Structural Biology

Observations on the behavior of vitreous ice at approximately 82 and approximately 12 K

Wright ER*, Iancu CV*, Tivol WF, Jensen GJ

Journal of Structural Biology

A comparison of liquid nitrogen and liquid helium as cryogens for electron cryotomography

Iancu CV, Wright ER, Heymann JB, Jensen GJ



Journal of Structural Biology

A “flip-flop” rotation stage for routine dual-axis electron cryotomography

Iancu CV*, Wright ER*, Benjamin J, Tivol WF, Dias DP, Murphy GE, Morrison RC, Heymann JB, Jensen GJ

Journal of Molecular Biology

Three-dimensional structure of HIV-1 virus-like particles by electron cryotomography

Benjamin J, Ganser-Pornillos BK, Tivol WF, Sundquist WI, Jensen GJ